BOLD fMRI of C-fiber mediated nociceptive processing in mouse brain in response to thermal stimulation of the forepaws

Bosshard, Simone C., Stuker, Florian, Von Deuster, Constantin, Schroeter, Aileen and Rudin, Markus (2015) BOLD fMRI of C-fiber mediated nociceptive processing in mouse brain in response to thermal stimulation of the forepaws. PLoS One, 10 5: e0126513-e0126513. doi:10.1371/journal.pone.0126513

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Author Bosshard, Simone C.
Stuker, Florian
Von Deuster, Constantin
Schroeter, Aileen
Rudin, Markus
Title BOLD fMRI of C-fiber mediated nociceptive processing in mouse brain in response to thermal stimulation of the forepaws
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2015-05-07
Year available 2015
Sub-type Article (original research)
DOI 10.1371/journal.pone.0126513
Open Access Status DOI
Volume 10
Issue 5
Start page e0126513
End page e0126513
Total pages 14
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2016
Language eng
Formatted abstract
Functional magnetic resonance imaging (fMRI) in rodents enables non-invasive studies of brain function in response to peripheral input or at rest. In this study we describe a thermal stimulation paradigm using infrared laser diodes to apply noxious heat to the forepaw of mice in order to study nociceptive processing. Stimulation at 45 and 46 degrees C led to robust BOLD signal changes in various brain structures including the somatosensory cortices and the thalamus. The BOLD signal amplitude scaled with the temperature applied but not with the area irradiated by the laser beam. To demonstrate the specificity of the paradigm for assessing nociceptive signaling we administered the quaternary lidocaine derivative QX-314 to the forepaws, which due to its positive charge cannot readily cross biological membranes. However, upon activation of TRPV1 channels following the administration of capsaicin the BOLD signal was largely abolished, indicative of a selective block of the C-fiber nociceptors due to QX-314 having entered the cells via the now open TRPV1 channels. This demonstrates that the cerebral BOLD response to thermal noxious paw stimulation is specifically mediated by C-fibers.
Keyword Cerebral blood flow
Lasting local anesthesia
Capsaicin receptor
Functional Mri
In vivo somatosensory stimulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Centre for Advanced Imaging Publications
 
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