Altered adhesion, proliferation and death in neural cultures from adults with schizophrenia

Feron, F., Perry, C., Hirning, M. H., McGrath, J. and Mackay-Sim, A. (1999) Altered adhesion, proliferation and death in neural cultures from adults with schizophrenia. Schizophrenia Research, 40 3: 211-218. doi:10.1016/S0920-9964(99)00055-9


Author Feron, F.
Perry, C.
Hirning, M. H.
McGrath, J.
Mackay-Sim, A.
Title Altered adhesion, proliferation and death in neural cultures from adults with schizophrenia
Journal name Schizophrenia Research   Check publisher's open access policy
ISSN 0929-9964
Publication date 1999-01-01
Sub-type Article (original research)
DOI 10.1016/S0920-9964(99)00055-9
Volume 40
Issue 3
Start page 211
End page 218
Total pages 8
Editor H. Nasrallah
L DeLisi
Place of publication New York
Publisher Elsevier
Collection year 2000
Language eng
Subject C3
321021 Psychiatry
730211 Mental health
Abstract The causes of schizophrenia are unknown, but there is evidence linking subtle deviations in neural development with schizophrenia. Embryonic brain development cannot be studied in an adult with schizophrenia, but neurogenesis and early events in neuronal differentiation can be investigated throughout adult life in the human olfactory epithelium. Our past research has demonstrated that neuronal cultures can be derived from biopsy of the human adult olfactory epithelium. In the present study, we examined mechanisms related to neurogenesis and neuronal differentiation in adults with schizophrenia versus well controls. Forty biopsies were collected under local anaesthesia from ten individuals with DSM III-R schizophrenia and ten age- and sex-matched well controls. All patients, except one, were receiving antipsychotic medication at the time of the biopsy, Immunostaining for neuronal markers indicated that neurogenesis occurred in the biopsies from both patients and controls since all contained cells expressing tubulin and/or olfactory marker protein. The major findings of this study are: 1. biopsies from patients with schizophrenia showed a significantly reduced ability to attach to the culture slide: 29.9% of patient biopsies attached compared to 73.5% of control biopsies; 2. biopsies from patients with schizophrenia had a significantly greater proportion of cells undergoing mitosis: 0.69% in the patients compared to 0.29% in the controls; and 3. dopamine (10 mu M) significantly increased the proportion of apoptotic cells in the control cultures but significantly decreased the proportion in patients' cultures. (C) 1999 Elsevier Science B.V. All rights reserved.
Keyword Psychiatry
Adhesion
Biopsies
Cell Cycle
Dopamine
Olfactory Epithelium
Schizophrenia
In-vitro
Cell
Fibroblasts
Fibronectin
Molecule
Deficits
Dopamine
Disorder
Growth
Cortex
Q-Index Code C3

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Psychology Publications
 
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Created: Mon, 13 Aug 2007, 21:29:16 EST