Three-dimensional imaging with optical tweezers

Friese, M. E. J., Truscott, A. G., Rubinsztein-Dunlop, H. and Heckenberg, N. R. (1999) Three-dimensional imaging with optical tweezers. Applied Optics, 38 31: 6597-6603. doi:10.1364/AO.38.006597


Author Friese, M. E. J.
Truscott, A. G.
Rubinsztein-Dunlop, H.
Heckenberg, N. R.
Title Three-dimensional imaging with optical tweezers
Journal name Applied Optics   Check publisher's open access policy
ISSN 0003-6935
1539-4522
Publication date 1999-11-01
Sub-type Article (original research)
DOI 10.1364/AO.38.006597
Volume 38
Issue 31
Start page 6597
End page 6603
Total pages 7
Place of publication Washington, D.C.
Publisher Optical Society of America
Language eng
Subject C1
02 Physical Sciences
020604 Quantum Optics
Abstract We demonstrate a three-dimensional scanning probe microscope in which the extremely soft spring of an optical tweezers trap is used. Feedback control of the instrument based on backscattered light levels allows three-dimensional imaging of microscopic samples in an aqueous environment. Preliminary results with a 2-mu m-diameter spherical probe indicate that features of approximately 200 nm can be resolved, with a sensitivity of 5 nm in the height measurement. The theoretical resolution is limited by the probe dimensions. (C) 1999 Optical Society of America.
Keyword Optics
Force Microscope
Trap
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Physical Sciences Publications
 
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Created: Mon, 13 Aug 2007, 21:27:06 EST