Structural hysteresis and hierarchy in adsorbed glycoproteins

Horvath, Robert, McColl, James, Yakubov, Gleb E. and Ramsden, Jeremy J. (2008) Structural hysteresis and hierarchy in adsorbed glycoproteins. Journal of Chemical Physics, 129 7: . doi:10.1063/1.2968127

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Author Horvath, Robert
McColl, James
Yakubov, Gleb E.
Ramsden, Jeremy J.
Title Structural hysteresis and hierarchy in adsorbed glycoproteins
Journal name Journal of Chemical Physics   Check publisher's open access policy
ISSN 0021-9606
Publication date 2008-08-21
Year available 2008
Sub-type Article (original research)
DOI 10.1063/1.2968127
Open Access Status File (Publisher version)
Volume 129
Issue 7
Total pages 4
Place of publication Melville, NY, United States
Publisher A I P Publishing LLC
Language eng
Formatted abstract
The adsorption and desorption of the giant heavily glycosylated protein mucin from solutions of different bulk concentrations have been followed at the nanometer scale using high resolution molecular microscopy based on optical waveguide lightmode spectroscopy. Modeling the layer as a uniaxial thin film allowed the in situ determination of adsorbed mass, mean layer thickness, and structural anisotropy. These parameters manifest highly significant adsorption-desorption hysteresis, indicating at least two dominant glycoprotein conformational types (i.e., molecular states, structurally and kinetically distinguishable). One of them is proposed to be a conformationally extended state that engenders uniaxial symmetry and dominates layers generated from low bulk concentrations. The revealed structure and the mechanism by which it is formed are postulated to be a general feature of the self-assembly of large glycoproteins. We expect that, inter alia, this knowledge will be relevant for understanding the extraordinary effectiveness of mucin thin films as boundary lubricants.
Keyword Chemistry, Physical
Physics, Atomic, Molecular & Chemical
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
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Created: Fri, 15 May 2015, 02:42:13 EST by Gleb Yakubov on behalf of Learning and Research Services (UQ Library)