Cell nanomechanics and focal adhesions are regulated by retinol and conjugated linoleic acid in a dose-dependent manner

Silberberg, Yaron R., Yakubov, Gleb E., Horton, Michael A. and Pelling, Andrew E. (2009) Cell nanomechanics and focal adhesions are regulated by retinol and conjugated linoleic acid in a dose-dependent manner. Nanotechnology, 20 28: . doi:10.1088/0957-4484/20/28/285103


Author Silberberg, Yaron R.
Yakubov, Gleb E.
Horton, Michael A.
Pelling, Andrew E.
Title Cell nanomechanics and focal adhesions are regulated by retinol and conjugated linoleic acid in a dose-dependent manner
Journal name Nanotechnology   Check publisher's open access policy
ISSN 0957-4484
1361-6528
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1088/0957-4484/20/28/285103
Volume 20
Issue 28
Total pages 9
Place of publication Temple Way, Bristol, United Kingdom
Publisher Institute of Physics Publishing
Language eng
Abstract Retinol and conjugated linoleic acid (CLA) have previously been shown to have an important role in gene expression and various cellular processes, including differentiation, proliferation and cell death. In this study we have investigated the effect of retinol and CLA, both individually and in combination, on the intracellular cytoskeleton, focal adhesions (FAs) and the nanomechanical properties of 3T3 fibroblasts. We observed a dose-dependent decrease in the formation of FAs following treatment with either compound, which was directly correlated to an increase in cell height (>30%) and a decrease in the measured Young's modulus (∼28%). Furthermore, treatments with both compounds demonstrated an increased effect and led to a reduction of>70% in the average number of FAs per cell and a decrease of >50% in average cell stiffness. These data reveal that retinol and CLA disrupt FA formation, leading to an increase in cell height and a significant decrease in stiffness. These results may broaden our understanding of the interplay between cell nanomechanics and cellular contact with the external microenvironment, and help to shed light on the important role of retinoids and CLA in health and disease.
Keyword Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Science & Technology - Other Topics
Materials Science
Physics
MATERIALS SCIENCE, MULTIDISCIPLINARY
NANOSCIENCE & NANOTECHNOLOGY
PHYSICS, APPLIED
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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Created: Fri, 15 May 2015, 02:36:26 EST by Gleb Yakubov on behalf of School of Chemical Engineering