TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice

Zhang, Hui, Gou, Feng, Zhang, Jinshan, Liu, Wenshan, Li, Qianqian, Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2015) TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice. Plant Biotechnology Journal, 14 1: 186-194. doi:10.1111/pbi.12372

Author Zhang, Hui
Gou, Feng
Zhang, Jinshan
Liu, Wenshan
Li, Qianqian
Mao, Yanfei
Botella, Jose Ramon
Zhu, Jian-Kang
Title TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7652
Publication date 2015-04-13
Year available 2015
Sub-type Article (original research)
DOI 10.1111/pbi.12372
Open Access Status DOI
Volume 14
Issue 1
Start page 186
End page 194
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing Ltd
Language eng
Subject 1305 Biotechnology
1102 Agronomy and Crop Science
1110 Plant Science
Abstract CRISPR/Cas9 and TALEN are currently the two systems of choice for genome editing. We have studied the efficiency of the TALEN system in rice as well as the nature and inheritability of TALEN-induced mutations and found important features of this technology. The N287C230 TALEN backbone resulted in low mutation rates (0–6.6%), but truncations in its C-terminal domain dramatically increased efficiency to 25%. In most transgenic T0 plants, TALEN produced a single prevalent mutation accompanied by a variety of low-frequency mutations. For each independent T0 plant, the prevalent mutation was present in most tissues within a single tiller as well as in all tillers examined, suggesting that TALEN-induced mutations occurred very early in the development of the shoot apical meristem. Multigenerational analysis showed that TALEN-induced mutations were stably transmitted to the T1 and T2 populations in a normal Mendelian fashion. In our study, the vast majority of TALEN-induced mutations (~81%) affected multiple bases and ~70% of them were deletions. Our results contrast with published reports for the CRISPR/Cas9 system in rice, in which the predominant mutations affected single bases and deletions accounted for only 3.3% of the overall mutations.
Keyword TALEN
Targeted genome editing
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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