Pre-coating layered double hydroxide nanoparticles with albumin to improve colloidal stability and cellular uptake

Gu, Zi, Zuo, Hualia, Li, Li, Wu, Aihua and Xu, Zhi Ping (2015) Pre-coating layered double hydroxide nanoparticles with albumin to improve colloidal stability and cellular uptake. Journal of Materials Chemistry B, 3 16: 3331-3339. doi:10.1039/c5tb00248f


Author Gu, Zi
Zuo, Hualia
Li, Li
Wu, Aihua
Xu, Zhi Ping
Title Pre-coating layered double hydroxide nanoparticles with albumin to improve colloidal stability and cellular uptake
Journal name Journal of Materials Chemistry B   Check publisher's open access policy
ISSN 2050-750X
2050-7518
Publication date 2015-04-28
Year available 2015
Sub-type Article (original research)
DOI 10.1039/c5tb00248f
Open Access Status Not Open Access
Volume 3
Issue 16
Start page 3331
End page 3339
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher RSC Publications
Collection year 2016
Language eng
Abstract One of the major challenges for nanoparticles to be used as a drug/gene delivery platform is their tendency to aggregate in electrolyte solution (physiological environment). The present work introduced the albumin pre-coating strategy that effectively prevented inorganic layered double hydroxide (LDH) nanoparticles with different sizes and interlayer anions from aggregation in phosphate buffer saline and cell culture medium solutions. We found that the key factors influencing the colloidal stability of albumin-coated LDHs included (1) the sequence and speed of reagent addition during the pre-coating process, (2) the albumin/LDH mass ratio, (3) the LDH particle size, and (4) anions intercalated in the LDH. Approximately, LDH nanoparticles with the size of 110 nm were well stabilised at the albumin/LDH mass ratio of 5 : 2 when LDH suspension was added into albumin solution dropwise with vigorous stirring. The albumin pre-coating also enhanced cellular uptake of LDH nanoparticles in Chinese hamster ovary cell culture. The configuration, affinity and adsorption isotherm of albumin on LDH nanoparticles were further investigated. The results in this work imply that the albumin-coating strategy is a potential method to prevent LDH nanoparticle aggregation in in vivo drug/gene delivery.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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