In vitro comparison of cryopreserved and liquid platelets: Potential clinical implications

Johnson L., Reade M.C., Hyland R.A., Tan S. and Marks D.C. (2015) In vitro comparison of cryopreserved and liquid platelets: Potential clinical implications. Transfusion, 55 4: 838-847. doi:10.1111/trf.12915

Author Johnson L.
Reade M.C.
Hyland R.A.
Tan S.
Marks D.C.
Title In vitro comparison of cryopreserved and liquid platelets: Potential clinical implications
Journal name Transfusion   Check publisher's open access policy
ISSN 1537-2995
Publication date 2015-04-01
Year available 2014
Sub-type Article (original research)
DOI 10.1111/trf.12915
Open Access Status Not yet assessed
Volume 55
Issue 4
Start page 838
End page 847
Total pages 10
Place of publication Hoboken, NJ, U.S.A.
Publisher Blackwell Publishing Inc.
Language eng
Subject 2720 Hematology
2403 Immunology
2723 Immunology and Allergy
Abstract Background Platelet (PLT) concentrates can be cryopreserved in dimethyl sulfoxide (DMSO) and stored at -80C for 2 years. These storage conditions improve availability in both rural and military environments. Previous phenotypic and in vitro studies of cryopreserved PLTs are limited by comparison to fresh liquid-stored PLTs, rather than PLTs stored over their clinically relevant shelf life. Further, nothing is known of the effect of reconstituting cryopreserved PLTs in plasma stored at a variety of clinically relevant temperatures. Study Design and Methods Apheresis PLTs were either stored at room temperature for 5 days or cryopreserved at -80C with 5% DMSO. Cryopreserved PLTs were thawed at 37C and reconstituted in plasma (stored at different temperatures) and compared to fresh and expired liquid-stored PLTs. In vitro assays were performed to assess glycoprotein expression, PLT activity, microparticle content, and function. Results Compared to liquid PLTs over storage, cryopreserved PLTs had reduced expression of the key glycoprotein receptors GPIbα and GPIIb. However, the proportion of PLTs expressing activation markers CD62P and CD63 was similar between cryopreserved and liquid-stored PLTs at expiry. Cryopreserved PLT components contained significantly higher numbers of phosphatidylserine- and tissue factor-positive microparticles than liquid-stored PLTs, and these microparticles reduced the time to clot formation and increased thrombin generation. Conclusion There are distinct differences between cryopreserved and liquid-stored PLTs. Cryopreserved PLTs also have an enhanced hemostatic activity. Knowledge of these in vitro differences will be essential to understanding the outcomes of a clinical trial comparing cryopreserved PLTs and liquid PLTs stored for various durations.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
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