IgE+ B cells are scarce, but allergen-specific B cells with a memory phenotype circulate in patients with allergic rhinitis

Wong, K.J, Timbrell, Victoria L., Xi, Y, Upham, J.W, Collins, A.M and Davies, J.M (2015) IgE+ B cells are scarce, but allergen-specific B cells with a memory phenotype circulate in patients with allergic rhinitis. Allergy: European Journal of Allergy and Clinical Immunology, 70 4: 420-428. doi:10.1111/all.12563


Author Wong, K.J
Timbrell, Victoria L.
Xi, Y
Upham, J.W
Collins, A.M
Davies, J.M
Title IgE+ B cells are scarce, but allergen-specific B cells with a memory phenotype circulate in patients with allergic rhinitis
Journal name Allergy: European Journal of Allergy and Clinical Immunology   Check publisher's open access policy
ISSN 1398-9995
0105-4538
Publication date 2015-04-01
Year available 2015
Sub-type Article (original research)
DOI 10.1111/all.12563
Open Access Status Not yet assessed
Volume 70
Issue 4
Start page 420
End page 428
Total pages 9
Place of publication Chichester, United Kingdom
Publisher Wiley-Blackwell Publishing Ltd
Language eng
Subject 2723 Immunology and Allergy
2403 Immunology
Abstract Background Despite the critical role of immunoglobulin E (IgE) in allergy, circulating IgE+ B cells are scarce. Here, we describe in patients with allergic rhinitis B cells with a memory phenotype responding to a prototypic aeroallergen. Methods Fifteen allergic rhinitis patients with grass pollen allergy and 13 control subjects were examined. Blood mononuclear cells stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) were cultured with Bahia grass pollen. Proliferation and phenotype were assessed by multicolour flow cytometry. Results In blood of allergic rhinitis patients with high serum IgE to grass pollen, most IgE cells were CD123+ HLA-DR basophils, with IgE for the major pollen allergen (Pas n 1). Both B and T cells from pollen-allergic donors showed higher proliferation to grass pollen than nonallergic donors (P = 0.002, and 0.010, respectively), whereas responses to vaccine antigens and mitogen did not differ between groups. Allergen-driven B cells that divided rapidly (CD19 CD3 CFSE) showed higher CD27 (P = 0.008) and lower CD19 (P = 0.004) and CD20 (P = 0.004) expression than B cells that were slow to respond to allergen (CD19 CD3 CFSE). Moreover, rapidly dividing allergen-driven B cells (CD19 CFSE CD27) showed higher expression of the plasmablast marker CD38 compared with B cells (CD19 CFSE CD27) that were slow to divide. Conclusion Patients with pollen allergy but not control donors have a population of circulating allergen-specific B cells with the phenotype and functional properties of adaptive memory B-cell responses. These cells could provide precursors for allergen-specific IgE production upon allergen re-exposure.
Formatted abstract
Background

Despite the critical role of immunoglobulin E (IgE) in allergy, circulating IgE+ B cells are scarce. Here, we describe in patients with allergic rhinitis B cells with a memory phenotype responding to a prototypic aeroallergen.

Methods

Fifteen allergic rhinitis patients with grass pollen allergy and 13 control subjects were examined. Blood mononuclear cells stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) were cultured with Bahia grass pollen. Proliferation and phenotype were assessed by multicolour flow cytometry.

Results

In blood of allergic rhinitis patients with high serum IgE to grass pollen, most IgEhi cells were CD123+ HLA-DR basophils, with IgE for the major pollen allergen (Pas n 1). Both B and T cells from pollen-allergic donors showed higher proliferation to grass pollen than nonallergic donors (P = 0.002, and 0.010, respectively), whereas responses to vaccine antigens and mitogen did not differ between groups. Allergen-driven B cells that divided rapidly (CD19mid CD3 CFSElo) showed higher CD27 (P = 0.008) and lower CD19 (P = 0.004) and CD20 (P = 0.004) expression than B cells that were slow to respond to allergen (CD19hi CD3 CFSEmid). Moreover, rapidly dividing allergen-driven B cells (CD19mid CFSElo CD27hi) showed higher expression of the plasmablast marker CD38 compared with B cells (CD19hi CFSEmid CD27lo) that were slow to divide.

Conclusion

Patients with pollen allergy but not control donors have a population of circulating allergen-specific B cells with the phenotype and functional properties of adaptive memory B-cell responses. These cells could provide precursors for allergen-specific IgE production upon allergen re-exposure.
Keyword allergic rhinitis
B cells
grass pollen
immunoglobulin E
immunoglobulin E B cells
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID GNT 1043311
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
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