Molecular structure of glycogen in diabetic liver

Deng, Bin, Sullivan, Mitchell A., Li, Jialun, Tan, Xinle, Zhu, Chengjun, Schulz, Benjamin L. and Gilbert, Robert G. (2015) Molecular structure of glycogen in diabetic liver. Glycoconjugate Journal, 32 3-4: 113-118. doi:10.1007/s10719-015-9578-6


Author Deng, Bin
Sullivan, Mitchell A.
Li, Jialun
Tan, Xinle
Zhu, Chengjun
Schulz, Benjamin L.
Gilbert, Robert G.
Title Molecular structure of glycogen in diabetic liver
Journal name Glycoconjugate Journal   Check publisher's open access policy
ISSN 1573-4986
0282-0080
Publication date 2015-05-01
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s10719-015-9578-6
Open Access Status Not yet assessed
Volume 32
Issue 3-4
Start page 113
End page 118
Total pages 6
Place of publication New York, NY United States
Publisher Springer New York
Language eng
Abstract Liver glycogen (involved in maintaining blood-sugar levels) is a hyperbranched glucose polymer containing β particles (diameter ~20 nm), which can form composite α particles (diameter ~50–300 nm), and includes a small but significant amount of bound protein. Size distributions of glycogen from livers of healthy and diabetic mice were examined using size-exclusion chromatography with two separate eluents: aqueous eluent and dimethylsulfoxide (DMSO) eluent. Morphologies were examined with transmission electron microscopy. Diabetic glycogen (DG) exhibited many α particles in the mild water-based solvent, but in DMSO, which breaks H bonds, these degraded to β particles; α particles however were always present in healthy glycogen (HG). This DG fragility shows the binding of β into α particles is different in HG and DG. The diabetic α particle fragility may be involved with the uncontrolled blood-sugar release symptomatic of diabetes: small β particles degrade more easily to glucose than α particles. This has implications for diabetes management.
Keyword Glycogen
db/db mice
Size-exclusion chromatography
Type 2 diabetes
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP130102461
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
Centre for Nutrition and Food Sciences Publications
 
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