Evaluation of dot immunogold filtration assay (DIGFA) by recombinant protein EPC1 for anti- echinococcus granulosus IGG antibody

Kordafshari, Somayeh, Hosseini, Seyed Hossein, Jalousian, Fatemeh, Rajabibazl, Masoumeh, Jones, Malcolm and Etebar, Fazeleh (2015) Evaluation of dot immunogold filtration assay (DIGFA) by recombinant protein EPC1 for anti- echinococcus granulosus IGG antibody. Iranian Journal of Parasitology, 10 1: 30-38.

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Author Kordafshari, Somayeh
Hosseini, Seyed Hossein
Jalousian, Fatemeh
Rajabibazl, Masoumeh
Jones, Malcolm
Etebar, Fazeleh
Title Evaluation of dot immunogold filtration assay (DIGFA) by recombinant protein EPC1 for anti- echinococcus granulosus IGG antibody
Journal name Iranian Journal of Parasitology   Check publisher's open access policy
ISSN 1735-7020
2008-238X
Publication date 2015-01-01
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 10
Issue 1
Start page 30
End page 38
Total pages 9
Place of publication Tehran, Iran
Publisher Tehran University of Medical Sciences Publications
Language eng
Formatted abstract
Background: Cystic echinococcosis is an important zoonosis caused by the Echinococcus granulosus with a substantial impact in human and animal health in en­demic areas. The purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method.

Methods: Eight dogs were challenged with protoscoleces in order to have posi­tive echinococcosis serum and 2 two-month old puppies were used as uninfected controls. Colloidal gold was prepared by controlled reduction of a boiling solu­tion of chloroauric acid (H [AuCl4]) with sodium citrate and labeled with recombi­nant EPC1. Dot immunogold filtration assay (DIGFA) was developed by coating rEPC1 labeled colloidal gold on nitrocellulose membrane. The canine sera, taken three times including, 15, 28 and 35 days post infection were tested. A total of 30 serum samples including 24 sera from 8 infected dogs and 6 sera from 2 puppies were comparatively detected with both DIGFA and ELISA.

Results: Gold labeled antigen, showed a dark purple dot with agglutination parti­cles in positive sera and light purple dot without agglutination in negative sera. Among 30 serum samples, 23 were positive and 7 were negative with DIGFA and 24 were positive, 6 were negative with ELISA.

Conclusion: DIGFA as a rapid and simple procedure could be utilized in quickly diagnosis of echinococcosis.
Keyword Dot immunogold filtration assay
Echinococcus granulosus
Recombinant EPC1
Serodiagnosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Veterinary Science Publications
 
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