Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice

Francois, Sylvia, Vidick, Sarah, Sarlet, Mickael, Desmecht, Daniel, Drion, Pierre, Stevenson, Philip G., Vanderplasschen, Alain and Gillet, Laurent (2013) Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice. PLoS Pathogens, 9 4: e1003292-e1003292. doi:10.1371/journal.ppat.1003292


Author Francois, Sylvia
Vidick, Sarah
Sarlet, Mickael
Desmecht, Daniel
Drion, Pierre
Stevenson, Philip G.
Vanderplasschen, Alain
Gillet, Laurent
Title Illumination of murine gammaherpesvirus-68 cycle reveals a sexual transmission route from females to males in laboratory mice
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7366
1553-7374
Publication date 2013-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1003292
Open Access Status DOI
Volume 9
Issue 4
Start page e1003292
End page e1003292
Total pages 15
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Formatted abstract
Transmission is a matter of life or death for pathogen lineages and can therefore be considered as the main motor of their evolution. Gammaherpesviruses are archetypal pathogenic persistent viruses which have evolved to be transmitted in presence of specific immune response. Identifying their mode of transmission and their mechanisms of immune evasion is therefore essential to develop prophylactic and therapeutic strategies against these infections. As the known human gammaherpesviruses, Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus are host-specific and lack a convenient in vivo infection model; related animal gammaherpesviruses, such as murine gammaherpesvirus-68 (MHV-68), are commonly used as general models of gammaherpesvirus infections in vivo. To date, it has however never been possible to monitor viral excretion or virus transmission of MHV-68 in laboratory mice population. In this study, we have used MHV-68 associated with global luciferase imaging to investigate potential excretion sites of this virus in laboratory mice. This allowed us to identify a genital excretion site of MHV-68 following intranasal infection and latency establishment in female mice. This excretion occurred at the external border of the vagina and was dependent on the presence of estrogens. However, MHV-68 vaginal excretion was not associated with vertical transmission to the litter or with horizontal transmission to female mice. In contrast, we observed efficient virus transmission to naïve males after sexual contact. In vivo imaging allowed us to show that MHV-68 firstly replicated in penis epithelium and corpus cavernosum before spreading to draining lymph nodes and spleen. All together, those results revealed the first experimental transmission model for MHV-68 in laboratory mice. In the future, this model could help us to better understand the biology of gammaherpesviruses and could also allow the development of strategies that could prevent the spread of these viruses in natural populations.
Keyword Microbiology
Parasitology
Virology
Microbiology
Parasitology
Virology
MICROBIOLOGY
PARASITOLOGY
VIROLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID D-09/11
F.4510.10
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Mon, 23 Mar 2015, 21:32:54 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences