Binary vectors for sense and antisense expression of arabidopsis ESTs

Mylne, J and Botella, JR (1998) Binary vectors for sense and antisense expression of arabidopsis ESTs. Plant Molecular Biology Reporter, 16 3: 257-262. doi:10.1023/A:1007520410190


Author Mylne, J
Botella, JR
Title Binary vectors for sense and antisense expression of arabidopsis ESTs
Journal name Plant Molecular Biology Reporter   Check publisher's open access policy
ISSN 0735-9640
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.1023/A:1007520410190
Open Access Status
Volume 16
Issue 3
Start page 257
End page 262
Total pages 6
Place of publication PISCATAWAY
Publisher TRANSACTION PERIOD CONSORTIUM
Language eng
Abstract Our laboratory is interested in devising methods to identify functions for the vast numbers of arabidopsis genes now available. For this purpose, we have constructed a set of binary vectors that will allow the quick production of transgenic arabidopsis plants containing either sense or antisense copies of EST clones obtained from the PRL2 library. These vectors are based on the pSLJ series containing the bialophos resistance (BAR) gene that confers resistance to the herbicide BASTA. Tn addition, our vectors contain a 35S CaMV promoter-polylinker-nos terminator cassette that allows the direct cloning of arabidopsis ESTs in either antisense (pAOV and pAOV2) or sense (pSOV and pSOV2) orientation. We also describe the construction of two additional vectors conferring BASTA resistance and containing the pBluescript polylinker in both orientations inserted between the 35S CaMV promoter and nos terminator (pKMB and pSMB).
Keyword Biochemical Research Methods
Plant Sciences
Antisense Constructs
Arabidopsis
Binary Vector
Est
Herbicide Resistance
Transgenic
Sense Constructs
Herbicide-resistance
Plants
Transformation
Genes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 13 Aug 2007, 20:54:37 EST