Micronucleus formation by single and mixed heavy metals/loids and PAH compounds in HepG2 cells

Peng, Cheng, Muthusamy, Sasikumar, Xia, Qing, Lal, Vincent, Denison, Michael S. and Ng, Jack C. (2015) Micronucleus formation by single and mixed heavy metals/loids and PAH compounds in HepG2 cells. Mutagenesis, 30 5: 593-602. doi:10.1093/mutage/gev021


Author Peng, Cheng
Muthusamy, Sasikumar
Xia, Qing
Lal, Vincent
Denison, Michael S.
Ng, Jack C.
Title Micronucleus formation by single and mixed heavy metals/loids and PAH compounds in HepG2 cells
Journal name Mutagenesis   Check publisher's open access policy
ISSN 0267-8357
1464-3804
Publication date 2015-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.1093/mutage/gev021
Open Access Status Not Open Access
Volume 30
Issue 5
Start page 593
End page 602
Total pages 10
Place of publication Oxford United Kingdom
Publisher Oxford University Press
Language eng
Abstract Humans and other organisms are exposed to multi-chemical mixtures including commonly found carcinogens such as polycyclic aromatic hydrocarbons (PAHs) and heavy metal/loids. The joint effects of these chemicals as beyond the binary mixtures have not been well characterised. In this study, we evaluated the combined genotoxicity of mixtures of PAHs and heavy metal/loids containing benzo(a)pyrene (B[a]P), naphthalene (Nap), phenanthrene (Phe), pyrene (Pyr), arsenic (As), cadmium (Cd) and chromium (Cr) using in vitro micronucleus (MN) test in HepG2 cells. The induction of aryl hydrocarbon receptor (AhR) by single and mixed PAHs was also measured. The results indicated that individual and mixed Nap, Phe and Pyr did not induce significant MN frequencies. PAHs mixture containing B[a]P and B[a]P alone caused significant but similar level of MN frequencies. The same pattern was found in their AhR induction. Individual metal/loids induced significant cytostasis and MN formation of which Cd was found the most potent inducer. Mixture of metal/loids caused higher frequency of MN suggesting a possible additive effect among metal/loids. In addition, binary mixture of metal/loids and B[a]P, namely As/B[a]P, Cd/B[a]P and Cr/B[a]P, increased MN formation. Mixture of Cd and B[a]P induced the highest level of MN. Exposure of cells to the mixture containing B[a]P and Cd/Cr/As at lower concentration (0.25 µM) resulted in significant MN frequency, the level of which was equal to that by Cd/B[a]P at 1.0 µM. The results of the study suggested that an additive effect may exist between PAHs and heavy metal/loids in a compound- and concentration-dependent manner. The compounds with highest potencies of genotoxicity in the mixture seem dominant as driving sources in the final combined genotoxicity of PAHs and heavy metal/loids.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published online: June 17, 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 10 Mar 2015, 22:09:35 EST by Robyne Anderson on behalf of National Res Centre For Environmental Toxicology