The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells

Phuyal, Santosh, Skotland, Tore, Hessvik, Nina Pettersen, Simolin, Helena, Overbye, Anders, Brech, Andreas, Parton, Robert G., Ekroos, Kim, Sandvig, Kirsten and Llorente, Alicia (2015) The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells. Journal of Biological Chemistry, 290 7: 4225-4237. doi:10.1074/jbc.M114.593962

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ352825_OA.pdf Full text (open access) application/pdf 3.35MB 0

Author Phuyal, Santosh
Skotland, Tore
Hessvik, Nina Pettersen
Simolin, Helena
Overbye, Anders
Brech, Andreas
Parton, Robert G.
Ekroos, Kim
Sandvig, Kirsten
Llorente, Alicia
Title The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 1083-351X
Publication date 2015-02-13
Year available 2014
Sub-type Article (original research)
DOI 10.1074/jbc.M114.593962
Open Access Status File (Publisher version)
Volume 290
Issue 7
Start page 4225
End page 4237
Total pages 13
Place of publication Rockville, MD United States
Publisher American Society for Biochemistry and Molecular Biology
Collection year 2015
Language eng
Abstract Exosomes are vesicles released by cells after fusion of multivesicular bodies with the plasma membrane. In this study, we have investigated whether ether lipids affect the release of exosomes in PC-3 cells. To increase the cellular levels of ether lipids, the ether lipid precursor hexadecylglycerol was added to cells. Lipidomic analysis showed that this compound was in fact able to double the cellular levels of ether lipids in these cells. Furthermore, increased levels of ether lipids were also found in exosomes released by cells containing high levels of these lipids. Interestingly, as measured by nanoparticle tracking analysis, cells containing high levels of ether lipids released more exosomes than control cells, and these exosomes were similar in size to control exosomes. Moreover, silver staining and Western blot analyses showed that the protein composition of exosomes released in the presence of hexadecylglycerol was changed; the levels of some proteins were increased, and the levels of others were reduced. In conclusion, this study clearly shows that an increase in cellular ether lipids is associated with changes in the release and composition of exosomes.
Keyword Extracellular vesicles
Lipid
Lipid ether
Membrane lipid
Membrane transport
Exosome release
Exosomes
High throughput quantitative lipidomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 17 Dec 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 03 Mar 2015, 10:25:11 EST by System User on behalf of Institute for Molecular Bioscience