High-throughput screening and small animal models, where are we?

Giacomotto, Jean and Segalat, Laurent (2010) High-throughput screening and small animal models, where are we?. British Journal of Pharmacology, 160 2: 204-216. doi:10.1111/j.1476-5381.2010.00725.x


Author Giacomotto, Jean
Segalat, Laurent
Title High-throughput screening and small animal models, where are we?
Journal name British Journal of Pharmacology   Check publisher's open access policy
ISSN 0007-1188
1476-5381
Publication date 2010-01-01
Year available 2010
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1111/j.1476-5381.2010.00725.x
Open Access Status Not yet assessed
Volume 160
Issue 2
Start page 204
End page 216
Total pages 13
Place of publication Chichester, West Sussex, United Kingdom
Publisher John Wiley & Sons
Language eng
Abstract Current high-throughput screening methods for drug discovery rely on the existence of targets. Moreover, most of the hits generated during screenings turn out to be invalid after further testing in animal models. To by-pass these limitations, efforts are now being made to screen chemical libraries on whole animals. One of the most commonly used animal model in biology is the murine model Mus musculus. However, its cost limit its use in large-scale therapeutic screening. In contrast, the nematode Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and the fish Danio rerio are gaining momentum as screening tools. These organisms combine genetic amenability, low cost and culture conditions that are compatible with large-scale screens. Their main advantage is to allow high-throughput screening in a whole-animal context. Moreover, their use is not dependent on the prior identification of a target and permits the selection of compounds with an improved safety profile. This review surveys the versatility of these animal models for drug discovery and discuss the options available at this day.
Keyword Caenorhabditis elegans
Chemical genetics
Danio rerio
Disease
Drosophila melanogaster
Drug discovery
Drug target
High throughput screening
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Wed, 25 Feb 2015, 21:31:44 EST by Anthony Yeates on behalf of Institute for Molecular Bioscience