Ultrafast cooling reveals microsecond-scale biomolecular dynamics

Polinkovsky, Mark E., Gambin, Yann, Banerjee, Priya R., Erickstad, Michael J., Groisman, Alex and Deniz, Ashok A. (2014) Ultrafast cooling reveals microsecond-scale biomolecular dynamics. Nature Communications, 5 5737: 1-7. doi:10.1038/ncomms6737

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Author Polinkovsky, Mark E.
Gambin, Yann
Banerjee, Priya R.
Erickstad, Michael J.
Groisman, Alex
Deniz, Ashok A.
Title Ultrafast cooling reveals microsecond-scale biomolecular dynamics
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2014-12-17
Year available 2014
Sub-type Article (original research)
DOI 10.1038/ncomms6737
Open Access Status File (Publisher version)
Volume 5
Issue 5737
Start page 1
End page 7
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1600 Chemistry
1300 Biochemistry, Genetics and Molecular Biology
3100 Physics and Astronomy
Abstract The temperature-jump technique, in which the sample is rapidly heated by a powerful laser pulse, has been widely used to probe the fast dynamics of folding of proteins and nucleic acids. However, the existing temperature-jump setups tend to involve sophisticated and expensive instrumentation, while providing only modest temperature changes of ~10–15 °C, and the temperature changes are only rapid for heating, but not cooling. Here we present a setup comprising a thermally conductive sapphire substrate with light-absorptive nano-coating, a microfluidic device and a rapidly switched moderate-power infrared laser with the laser beam focused on the nano-coating, enabling heating and cooling of aqueous solutions by ~50 °C on a 1-μs time scale. The setup is used to probe folding and unfolding dynamics of DNA hairpins after direct and inverse temperature jumps, revealing low-pass filter behaviour during periodic temperature variations.
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DP12010423
MCB 1121959
PHY 0750049
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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