Discrimination of pseudogene and parental gene DNA methylation using allelic bisulfite sequencing

Hesson, Luke B. and Ward, Robyn L. (2014). Discrimination of pseudogene and parental gene DNA methylation using allelic bisulfite sequencing. In Pseudogenes: functions and protocols (pp. 265-274) New York, NY United States: Springer. doi:10.1007/978-1-4939-0835-6_18


Author Hesson, Luke B.
Ward, Robyn L.
Title of chapter Discrimination of pseudogene and parental gene DNA methylation using allelic bisulfite sequencing
Title of book Pseudogenes: functions and protocols
Place of Publication New York, NY United States
Publisher Springer
Publication Year 2014
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-0835-6_18
Open Access Status Not Open Access
Year available 2014
Series Methods in Molecular Biology
ISBN 9781493908349
9781493908356
ISSN 1064-3745
Volume number 1167
Chapter number 18
Start page 265
End page 274
Total pages 10
Total chapters 21
Language eng
Formatted Abstract/Summary
Determining the methylation status of genes with pseudogenes can be technically challenging due to sequence homology. High sequence homology can result in the amplification of both pseudogene and parental gene alleles, potentially leading to data misinterpretation. Allelic bisulfite sequencing allows for detection of the methylation status of individual alleles at nucleotide resolution and represents the most reliable method for discriminating pseudogene and parental gene sequences. Here, we discuss important points that should be considered when investigating pseudogene and parental gene methylation status and we describe the method of allelic bisulfite sequencing, including assay design.
Keyword Pseudogene
Epigenetics
Methylation
Bisulfite
Sequencing
Tumor Suppressor Gene
Annotation
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
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Created: Thu, 12 Feb 2015, 21:37:53 EST by Ms Kate Rowe on behalf of Office of Deputy Vice-Chancellor (Research)