Rapid isolation of high-quality RNA from symbiotic dinoflagellates

Mylne, JS and Botella, JR (1998) Rapid isolation of high-quality RNA from symbiotic dinoflagellates. Phycologia, 37 4: 307-309. doi:10.2216/i0031-8884-37-4-307.1


Author Mylne, JS
Botella, JR
Title Rapid isolation of high-quality RNA from symbiotic dinoflagellates
Journal name Phycologia   Check publisher's open access policy
ISSN 0031-8884
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.2216/i0031-8884-37-4-307.1
Open Access Status
Volume 37
Issue 4
Start page 307
End page 309
Total pages 3
Place of publication LAWRENCE
Publisher INT PHYCOLOGICAL SOC
Language eng
Abstract This report details a reliable and efficient RNA extraction protocol for the symbiotic dinoflagellate Symbiodinium microadriaticum Freudenthal (Gymnodiniales, Dinophyceae). The method typically gives yields of 500 mu g total RNA from 0.4 g wet weight of algae, and, in comparison to current protocols, it is technically simple and less time consuming. This method isolates high-quality, intact RNA from in vine cultured as well as host-isolated cells, as demonstrated by spectrophotometry, gel electrophoresis, and northern analysis. The total RNA obtained was suitable for reverse transcription and PCR amplification of Symbiodinium cDNAs. We have successfully applied our method to isolate total RNA from a different dinoflagellate, Amphidinium carterae Hulburt (Gymnodiniales, Dinophyceae), found in symbiotic association with marine invertebrates.
Keyword Plant Sciences
Marine & Freshwater Biology
Nucleotide-sequence
Dna-sequence
Zooxanthellae
Diversity
Protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 13 Aug 2007, 20:41:45 EST