Synthetic protein switches: design principles and applications

Stein, Viktor and Alexandrov, Kirill (2015) Synthetic protein switches: design principles and applications. Trends in Biotechnology, 33 2: 101-110. doi:10.1016/j.tibtech.2014.11.010

Author Stein, Viktor
Alexandrov, Kirill
Title Synthetic protein switches: design principles and applications
Journal name Trends in Biotechnology   Check publisher's open access policy
ISSN 1879-3096
Publication date 2015-02-01
Year available 2014
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.tibtech.2014.11.010
Open Access Status
Volume 33
Issue 2
Start page 101
End page 110
Total pages 10
Place of publication Kidlington, Oxford, United Kingdom
Publisher Elsevier
Language eng
Subject 1305 Biotechnology
1502 Bioengineering
Abstract Protein switches are ubiquitous in biological signal transduction systems, enabling cells to sense and respond to a variety of molecular queues in a rapid, specific, and integrated fashion. Analogously, tailor-engineered protein switches with custom input and output functions have become invaluable research tools for reporting on distinct physiological states and actuating molecular functions in real time and in situ. Here, we analyze recent progress in constructing protein-based switches while assessing their potential in the assembly of defined signaling motifs. We anticipate such systems will ultimately pave the way towards a new generation of molecular diagnostics and facilitate the construction of artificial signaling systems that operate in parallel to the signaling machinery of a host cell for applications in synthetic biology.
Keyword Synthetic biology
Protein switches
Protein engineering
Molecular diagnostics
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 569652
Institutional Status UQ
Additional Notes Published online ahead of print 20 Dec 2014

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 25 times in Scopus Article | Citations
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