Are there functional P2X receptors on cell bodies in intact dorsal root ganglia of rats?

Stebbing, MJ, McLachlan, EM and Sah, P (1998) Are there functional P2X receptors on cell bodies in intact dorsal root ganglia of rats?. Neuroscience, 86 4: 1235-1244. doi:10.1016/S0306-4522(98)00127-4


Author Stebbing, MJ
McLachlan, EM
Sah, P
Title Are there functional P2X receptors on cell bodies in intact dorsal root ganglia of rats?
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
Publication date 1998-01-01
Sub-type Article (original research)
DOI 10.1016/S0306-4522(98)00127-4
Volume 86
Issue 4
Start page 1235
End page 1244
Total pages 10
Language eng
Abstract P2X purinoceptors have been suggested to participate in transduction of painful stimuli in nociceptive neurons. In the current experiments, ATP (1-10 mM), alpha,beta-methylene-ATP (10-30 mu M) and capsaicin (10 nM-1 mu M) were applied to neurons impaled with high resistance microelectrodes in rat dorsal root ganglia (L4 and L5) isolated in vitro together with the sciatic nerve and dorsal roots. The agonists were either bath applied or focally applied using a picospritzer. GABA (100 mu M) and 40-80 mM K+ solutions gave brisk responses when applied by either technique. Only three of 22 neurons with slowly conducting axons (C cells) showed evidence of P2X-purinoceptor-mediated responses. Only two of 13 cells which responded to capsaicin (putative nociceptors), and none of 29 cells with rapidly conducting axons (A cells), responded to the purinergic agonists. When acutely dissociated dorsal root ganglion cells were studied using patch-clamp techniques, all but four of 30 cells of all sizes responded with an inward current to either ATP or alpha,beta-methylene-ATP (both 100 mu M). Our data suggest that few sensory cell bodies in intact dorsal root ganglia express functional purinoceptors. (C) 1998 IBRO. Published by Elsevier Science Ltd.
Keyword Neurosciences
Atp
Alpha,beta-methylene-atp
Dorsal Root Ganglia
P2x Receptors
Capsaicin
Gated Ion Channels
Sensory Neurons
Guinea-pig
Electrophysiological Properties
Purinergic Receptors
Celiac Neurons
Atp
Nociception
Responses
Invitro
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Mon, 13 Aug 2007, 20:32:03 EST