Are there functional P2X receptors on cell bodies in intact dorsal root ganglia of rats?

Stebbing, MJ, McLachlan, EM and Sah, P (1998) Are there functional P2X receptors on cell bodies in intact dorsal root ganglia of rats?. Neuroscience, 86 4: 1235-1244. doi:10.1016/S0306-4522(98)00127-4

Author Stebbing, MJ
McLachlan, EM
Sah, P
Title Are there functional P2X receptors on cell bodies in intact dorsal root ganglia of rats?
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.1016/S0306-4522(98)00127-4
Open Access Status
Volume 86
Issue 4
Start page 1235
End page 1244
Total pages 10
Place of publication OXFORD
Language eng
Abstract P2X purinoceptors have been suggested to participate in transduction of painful stimuli in nociceptive neurons. In the current experiments, ATP (1-10 mM), alpha,beta-methylene-ATP (10-30 mu M) and capsaicin (10 nM-1 mu M) were applied to neurons impaled with high resistance microelectrodes in rat dorsal root ganglia (L4 and L5) isolated in vitro together with the sciatic nerve and dorsal roots. The agonists were either bath applied or focally applied using a picospritzer. GABA (100 mu M) and 40-80 mM K+ solutions gave brisk responses when applied by either technique. Only three of 22 neurons with slowly conducting axons (C cells) showed evidence of P2X-purinoceptor-mediated responses. Only two of 13 cells which responded to capsaicin (putative nociceptors), and none of 29 cells with rapidly conducting axons (A cells), responded to the purinergic agonists. When acutely dissociated dorsal root ganglion cells were studied using patch-clamp techniques, all but four of 30 cells of all sizes responded with an inward current to either ATP or alpha,beta-methylene-ATP (both 100 mu M). Our data suggest that few sensory cell bodies in intact dorsal root ganglia express functional purinoceptors. (C) 1998 IBRO. Published by Elsevier Science Ltd.
Keyword Neurosciences
Dorsal Root Ganglia
P2x Receptors
Gated Ion Channels
Sensory Neurons
Electrophysiological Properties
Purinergic Receptors
Celiac Neurons
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 36 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 13 Aug 2007, 20:32:03 EST