Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia

Goldspink, L. K., Mollinger, J. L., Barnes, T. S., Groves, M., Mahony, T. J. and Gibson, J. S. (2015) Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia. Veterinary Journal, 203 2: 239-243. doi:10.1016/j.tvjl.2014.12.008

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Author Goldspink, L. K.
Mollinger, J. L.
Barnes, T. S.
Groves, M.
Mahony, T. J.
Gibson, J. S.
Title Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia
Formatted title
Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia
Journal name Veterinary Journal   Check publisher's open access policy
ISSN 1532-2971
1090-0233
Publication date 2015-02-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.tvjl.2014.12.008
Open Access Status
Volume 203
Issue 2
Start page 239
End page 243
Total pages 5
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
This study investigated antimicrobial resistance traits, clonal relationships and epidemiology of I isolated from clinically affected cattle in Queensland and New South Wales, Australia. Isolates (n = 53) were subjected to antimicrobial susceptibility testing against six antimicrobial agents (ceftiofur, enrofloxacin, florfenicol, tetracycline, tilmicosin and tulathromycin) using disc diffusion and minimum inhibitory concentration (MIC) assays. Clonal relationships were assessed using repetitive sequence PCR and descriptive epidemiological analysis was performed. The H. somni isolates appeared to be geographically clonal, with 27/53 (47%) isolates grouping in one cluster from one Australian state. On the basis of disc diffusion, 34/53 (64%) isolates were susceptible to all antimicrobial agents tested; there was intermediate susceptibility to tulathromycin in 12 isolates, tilmicosin in seven isolates and resistance to tilmicosin in one isolate. Using MIC, all but one isolate was susceptible to all antimicrobial agents tested; the non-susceptible isolate was resistant to tetracycline, but this MIC result could not be compared to disc diffusion, since there are no interpretative guidelines for disc diffusion for H. somni against tetracycline. In this study, there was little evidence of antimicrobial resistance in H. somni isolates from Australian cattle. Disc diffusion susceptibility testing results were comparable to MIC results for most antimicrobial agents tested; however, results for isolates with intermediate susceptibility or resistance to tilmicosin and tulathromycin on disc diffusion should be interpreted with caution in the absence of MIC results.
Keyword Histophilus somni
Bovine respiratory disease
Antimicrobial susceptibility
Disc diffusion
Minimum inhibitory concentration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 12 Dec 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
School of Veterinary Science Publications
 
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