Divergent reprogramming routes lead to alternative stem-cell states

Tonge, Peter D., Corso, Andrew J., Monetti, Claudio, Hussein, Samer M. I., Puri, Mira C., Michael, Iacovos P., Li, Mira, Lee, Dong-Sung, Mar, Jessica C., Cloonan, Nicole, Wood, David L., Gauthier, Maely E., Korn, Othmar, Clancy, Jennifer L., Preiss, Thomas, Grimmond, Sean M., Shin, Jong-Yeon, Seo, Jeong-Sun, Wells, Christine A., Rogers, Ian M. and Nagy, Andras (2014) Divergent reprogramming routes lead to alternative stem-cell states. Nature, 516 7530: 192-197. doi:10.1038/nature14047

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Author Tonge, Peter D.
Corso, Andrew J.
Monetti, Claudio
Hussein, Samer M. I.
Puri, Mira C.
Michael, Iacovos P.
Li, Mira
Lee, Dong-Sung
Mar, Jessica C.
Cloonan, Nicole
Wood, David L.
Gauthier, Maely E.
Korn, Othmar
Clancy, Jennifer L.
Preiss, Thomas
Grimmond, Sean M.
Shin, Jong-Yeon
Seo, Jeong-Sun
Wells, Christine A.
Rogers, Ian M.
Nagy, Andras
Title Divergent reprogramming routes lead to alternative stem-cell states
Journal name Nature   Check publisher's open access policy
ISSN 0028-0836
Publication date 2014-12-01
Year available 2014
Sub-type Article (original research)
DOI 10.1038/nature14047
Open Access Status
Volume 516
Issue 7530
Start page 192
End page 197
Total pages 6
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
Pluripotency is defined by the ability of a cell to differentiate to the derivatives of all the three embryonic germ layers: ectoderm, mesoderm and endoderm. Pluripotent cells can be captured via the archetypal derivation of embryonic stem cells or via somatic cell reprogramming. Somatic cells are induced to acquire a pluripotent stem cell (iPSC) state through the forced expression of key transcription factors, and in the mouse these cells can fulfil the strictest of all developmental assays for pluripotent cells by generating completely iPSC-derived embryos and mice. However, it is not known whether there are additional classes of pluripotent cells, or what the spectrum of reprogrammed phenotypes encompasses. Here we explore alternative outcomes of somatic reprogramming by fully characterizing reprogrammed cells independent of preconceived definitions of iPSC states. We demonstrate that by maintaining elevated reprogramming factor expression levels, mouse embryonic fibroblasts go through unique epigenetic modifications to arrive at a stable, Nanog-positive, alternative pluripotent state. In doing so, we prove that the pluripotent spectrum can encompass multiple, unique cell states.
Keyword Self Renewal
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 10 December 2014

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Citation counts: TR Web of Science Citation Count  Cited 59 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 23 Jan 2015, 22:04:29 EST by Susan Allen on behalf of Institute for Molecular Bioscience