Efficient water soluble O-carboxymethyl chitosan nanocarrier for the delivery of curcumin to cancer cells

Anitha, A., Maya, S., Deepa, N., Chennazhi, K. P., Nair, S. V., Tamura, H. and Jayakumar, R. (2011) Efficient water soluble O-carboxymethyl chitosan nanocarrier for the delivery of curcumin to cancer cells. Carbohydrate Polymers, 83 2: 452-461. doi:10.1016/j.carbpol.2010.08.008


Author Anitha, A.
Maya, S.
Deepa, N.
Chennazhi, K. P.
Nair, S. V.
Tamura, H.
Jayakumar, R.
Title Efficient water soluble O-carboxymethyl chitosan nanocarrier for the delivery of curcumin to cancer cells
Journal name Carbohydrate Polymers   Check publisher's open access policy
ISSN 0144-8617
1879-1344
Publication date 2011-01-10
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.carbpol.2010.08.008
Open Access Status Not yet assessed
Volume 83
Issue 2
Start page 452
End page 461
Total pages 10
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Abstract Carboxymethyl chitosan derivatives are widely used for biomedical applications because of their non-toxic and biodegradable properties. Curcumin is a phytochemical with immense biological properties. But its hydrophobicity and poor oral bioavailability limits its application as a chemotherapeutic agent. To increase the oral bioavailability, we developed curcumin loaded O-CMC nanoparticles (curcumin-O-CMC Nps). The prepared nanoparticles were characterized by DLS, AFM, SEM, FT-IR, XRD and TG/DTA. Size analysis studies revealed spherical particles with mean diameter of about 150 ± 30 nm. Curcumin was entrapped in O-CMC with an efficiency of 87%. In vitro drug release profile was studied at 37 °C under different pHs (7.4 and 4.5) with and without lysozyme. Cytotoxicity studies by MTT assay indicated that curcumin-O-CMC Nps were toxic to cancer and non-toxic to normal cells. Cellular uptake of the curcumin-O-CMC Nps was analyzed by fluorescence microscopy and FACS. Overall these studies indicated O-CMC as a promising nanomatrix for drug delivery applications.
Keyword Carboxymethyl chitosan
Cellular uptake
Curcumin
Cytotoxicity
Drug delivery
Nanoformulation
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID BT/PR10882/NNT/28/142/2008
9/963(0005)2K10-EMR-I
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Thu, 22 Jan 2015, 23:57:45 EST by Anitha Sudheesh Kumar on behalf of School of Chemistry & Molecular Biosciences