LC-MS-Based Metabolomics Study of Marine Bacterial Secondary Metabolite and Antibiotic Production in Salinispora arenicola.

Bose, Utpal, Hewavitharana, Amitha K., Ng, Yi Kai, Shaw, Paul Nicholas, Fuerst, John A. and Hodson, Mark P. (2015) LC-MS-Based Metabolomics Study of Marine Bacterial Secondary Metabolite and Antibiotic Production in Salinispora arenicola.. Marine Drugs, 13 1: 249-266. doi:10.3390/md13010249


Author Bose, Utpal
Hewavitharana, Amitha K.
Ng, Yi Kai
Shaw, Paul Nicholas
Fuerst, John A.
Hodson, Mark P.
Title LC-MS-Based Metabolomics Study of Marine Bacterial Secondary Metabolite and Antibiotic Production in Salinispora arenicola.
Journal name Marine Drugs   Check publisher's open access policy
ISSN 1660-3397
Publication date 2015-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.3390/md13010249
Open Access Status DOI
Volume 13
Issue 1
Start page 249
End page 266
Total pages 18
Place of publication Basel, Switzerland
Publisher M D P I AG
Collection year 2015
Language eng
Formatted abstract
: An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A “chemical profile” link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time.
Keyword Salinispora arenicola
Salt Concentrations
Antibiotic
Metabolomics
Ssecondary metabolites
Liquid chromatography
Mass spectrometry
Multivariate analysis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 16 Jan 2015, 21:31:43 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences