Iron, copper and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress

Ribeiro, Thales P., Fernandes, Christiane, Melo, Karen V., Ferreira, Sarah S., Lessa, Josane A., Franco, Roberto W A., Schenk, Gerhard, Pereira, Marcos D. and Horn, Adolfo (2015) Iron, copper and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress. Free Radical Biology and Medicine, 80 67-76. doi:10.1016/j.freeradbiomed.2014.12.005

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Author Ribeiro, Thales P.
Fernandes, Christiane
Melo, Karen V.
Ferreira, Sarah S.
Lessa, Josane A.
Franco, Roberto W A.
Schenk, Gerhard
Pereira, Marcos D.
Horn, Adolfo
Title Iron, copper and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress
Formatted title
Iron, copper and manganese complexes with in vitro superoxide dismutase and/or catalase activities that keep Saccharomyces cerevisiae cells alive under severe oxidative stress
Journal name Free Radical Biology and Medicine   Check publisher's open access policy
ISSN 0891-5849
1873-4596
Publication date 2015-03-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.freeradbiomed.2014.12.005
Open Access Status File (Author Post-print)
Volume 80
Start page 67
End page 76
Total pages 10
Place of publication Philadelphia, PA United States
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
Due to their aerobic lifestyle, eukaryotic organisms have evolved different strategies to overcome oxidative stress. The recruitment of some specific metalloenzymes such as superoxide dismutases (SODs) and catalases (CATs) is of great importance for eliminating harmful reactive oxygen species (hydrogen peroxide and superoxide anion). Using the ligand HPClNOL {1-[bis(pyridin-2-ylmethyl)amino]-3-chloropropan-2-ol}, we have synthesized three coordination compounds containing iron(III), copper(II), and manganese(II) ions, which are also present in the active site of the above-noted metalloenzymes. These compounds were evaluated as SOD and CAT mimetics. The manganese and iron compounds showed both SOD and CAT activities, while copper showed only SOD activity. The copper and manganese in vitro SOD activities are very similar (IC50~0.4 μmol dm–3) and about 70-fold higher than those of iron. The manganese compound showed CAT activity higher than that of the iron species. Analyzing their capacity to protect Saccharomyces cerevisiae cells against oxidative stress (H2O2 and the O2•– radical), we observed that all compounds act as antioxidants, increasing the resistance of yeast cells mainly due to a reduction of lipid oxidation. Especially for the iron compound, the data indicate complete protection when wild-type cells were exposed to H2O2 or O2•– species. Interestingly, these compounds also compensate for both superoxide dismutase and catalase deficiencies; their antioxidant activity is metal ion dependent, in the order iron(III)>copper(II)>manganese(II). The protection mechanism employed by the complexes proved to be independent of the activation of transcription factors (such as Yap1, Hsf1, Msn2/Msn4) and protein synthesis. There is no direct relation between the in vitro and the in vivo antioxidant activities.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 13 Dec 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 16 Jan 2015, 21:12:39 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences