Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis

Wolfenstetter, Susanne, Chakravorty, David, Kula, Ryan, Urano, Daisuke, Trusov, Yuri, Sheahan, Michael B., McCurdy, David W., Assmann, Sarah M., Jones, Alan M. and Botella, Jose R. (2014) Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis. Plant Journal, 81 3: 388-398. doi:10.1111/tpj.12732


Author Wolfenstetter, Susanne
Chakravorty, David
Kula, Ryan
Urano, Daisuke
Trusov, Yuri
Sheahan, Michael B.
McCurdy, David W.
Assmann, Sarah M.
Jones, Alan M.
Botella, Jose R.
Title Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis
Journal name Plant Journal   Check publisher's open access policy
ISSN 1365-313X
0960-7412
Publication date 2014-12-22
Year available 2014
Sub-type Article (original research)
DOI 10.1111/tpj.12732
Open Access Status Not yet assessed
Volume 81
Issue 3
Start page 388
End page 398
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract Heterotrimeric G proteins are crucial for the perception of external signals and subsequent signal transduction in animal and plant cells. In both model systems, the complex comprises one Gα, one Gβ, and one Gγ subunit. However, in addition to the canonical Gγ subunits (class A), plants also possess two unusual, plant-specific classes of Gγ subunits (classes B and C) that have not yet been found in animals. These include Gγ subunits lacking the C–terminal CaaX motif (class B), which is important for membrane anchoring of the protein; the presence of such subunits gives rise to a flexible sub-population of Gβ/γ heterodimers that are not necessarily restricted to the plasma membrane. Plants also contain class C Gγ subunits, which are twice the size of canonical Gγ subunits, with a predicted transmembrane domain and a large cysteine-rich extracellular C–terminus. However, neither the presence of the transmembrane domain nor the membrane topology have been unequivocally demonstrated. Here, we provide compelling evidence that AGG3, a class C Gγ subunit of Arabidopsis, contains a functional transmembrane domain, which is sufficient but not essential for plasma membrane localization, and that the cysteine-rich C–terminus is extracellular.
Keyword Arabidopsis Gγ subunit 3, AGG3
Membrane topology
Heterotrimeric G protein
Arabidopsis thaliana
Type II membrane protein
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID R01GM065989
MCB-0723515
DE-FG02-05er15671
MCB-1121612
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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