Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee

Cristino, Alexandre S., Barchuk, Angel R., Freitas, Flavia C. P., Narayanan, Ramesh K., Biergans, Stephanie D., Zhao, Zhengyang, Simoes, Zila L. P., Reinhard, Judith and Claudianos, Charles (2014) Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee. Nature Communications, 5 1-11. doi:10.1038/ncomms6529

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Author Cristino, Alexandre S.
Barchuk, Angel R.
Freitas, Flavia C. P.
Narayanan, Ramesh K.
Biergans, Stephanie D.
Zhao, Zhengyang
Simoes, Zila L. P.
Reinhard, Judith
Claudianos, Charles
Title Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2014-11-20
Year available 2014
Sub-type Article (original research)
DOI 10.1038/ncomms6529
Open Access Status File (Publisher version)
Volume 5
Start page 1
End page 11
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Increasing evidence suggests small non-coding RNAs (ncRNAs) such as microRNAs (miRNAs) control levels of mRNA expression during experience-related remodelling of the brain. Here we use an associative olfactory learning paradigm in the honeybee Apis mellifera to examine gene expression changes in the brain during memory formation. Brain transcriptome analysis reveals a general downregulation of protein-coding genes, including asparagine synthetase and ​actin, and upregulation of ncRNAs. miRNA–mRNA network predictions together with PCR validation suggest miRNAs including ​miR-210 and ​miR-932 target the downregulated protein-coding genes. Feeding ​cholesterol-conjugated antisense RNA to bees results in the inhibition of ​miR-210 and of ​miR-932. Loss of ​miR-932 impairs long-term memory formation, but not memory acquisition. Functional analyses show that ​miR-932 interacts with ​Act5C, providing evidence for direct regulation of ​actin expression by an miRNA. An activity-dependent increase in ​miR-932 expression may therefore control ​actin-related plasticity mechanisms and affect memory formation in the brain.
Keyword Long term memory
Apis mellifera
Synaptic function
Mushroom bodies
Small RNAs
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
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