Towards a quantitative theory of epidermal calcium profile formation in unwounded skin

Adams, Matthew P., Mallet, Daniel G. and Pettet, Graeme J. (2015) Towards a quantitative theory of epidermal calcium profile formation in unwounded skin. PLoS One, 10 1: e0116751.1-e0116751.23. doi:10.1371/journal.pone.0116751

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Author Adams, Matthew P.
Mallet, Daniel G.
Pettet, Graeme J.
Title Towards a quantitative theory of epidermal calcium profile formation in unwounded skin
Journal name PLoS One   Check publisher's open access policy
ISSN 1544-9173
Publication date 2015-01-27
Sub-type Article (original research)
DOI 10.1371/journal.pone.0116751
Open Access Status DOI
Volume 10
Issue 1
Start page e0116751.1
End page e0116751.23
Total pages 23
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1100 Agricultural and Biological Sciences
Abstract We propose and mathematically examine a theory of calcium profile formation in unwounded mammalian epidermis based on: changes in keratinocyte proliferation, fluid and calcium exchange with the extracellular fluid during these cells’ passage through the epidermal sublayers, and the barrier functions of both the stratum corneum and tight junctions localised in the stratum granulosum. Using this theory, we develop a mathematical model that predicts epidermal sublayer transit times, partitioning of the epidermal calcium gradient between intracellular and extracellular domains, and the permeability of the tight junction barrier to calcium ions. Comparison of our model’s predictions of epidermal transit times with experimental data indicates that keratinocytes lose at least 87% of their volume during their disintegration to become corneocytes. Intracellular calcium is suggested as the main contributor to the epidermal calcium gradient, with its distribution actively regulated by a phenotypic switch in calcium exchange between keratinocytes and extracellular fluid present at the boundary between the stratum spinosum and the stratum granulosum. Formation of the extracellular calcium distribution, which rises in concentration through the stratum granulosum towards the skin surface, is attributed to a tight junction barrier in this sublayer possessing permeability to calcium ions that is less than 15 nm s−1 in human epidermis and less than 37 nm s−1 in murine epidermis. Future experimental work may refine the presented theory and reduce the mathematical uncertainty present in the model predictions
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
Official 2016 Collection
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
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Created: Fri, 09 Jan 2015, 22:05:19 EST by Matthew Adams on behalf of School of Chemical Engineering