Genome-wide identification of phenolic acid biosynthetic genes in Salvia miltiorrhiza

Wang, Bo, Sun, Wei, Li, Qiushi, Li, Ying, Luo, Hongmei, Song, Jingyuan, Sun, Chao, Qian, Jun, Zhu, Yingjie, Hayward, Alice, Xu, Haibin and Chen, Shilin (2014) Genome-wide identification of phenolic acid biosynthetic genes in Salvia miltiorrhiza. Planta, 241 3: 711-725. doi:10.1007/s00425-014-2212-1


Author Wang, Bo
Sun, Wei
Li, Qiushi
Li, Ying
Luo, Hongmei
Song, Jingyuan
Sun, Chao
Qian, Jun
Zhu, Yingjie
Hayward, Alice
Xu, Haibin
Chen, Shilin
Title Genome-wide identification of phenolic acid biosynthetic genes in Salvia miltiorrhiza
Formatted title
Genome-wide identification of phenolic acid biosynthetic genes in Salvia miltiorrhiza
Journal name Planta   Check publisher's open access policy
ISSN 1432-2048
0032-0935
Publication date 2014-12-04
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s00425-014-2212-1
Open Access Status Not yet assessed
Volume 241
Issue 3
Start page 711
End page 725
Total pages 15
Place of publication Heidelberg Germany
Publisher Springer
Language eng
Subject 1311 Genetics
1110 Plant Science
Abstract Twenty-nine genes related to phenolic acid biosynthesis were identified in the Salvia miltiorrhiza genome. Nineteen of these are described for the first time, with ten genes experimentally correlating to phenolic acid biosynthesis.
Formatted abstract
Main conclusion

Twenty-nine genes related to phenolic acid biosynthesis were identified in the Salvia miltiorrhiza genome. Nineteen of these are described for the first time, with ten genes experimentally correlating to phenolic acid biosynthesis.


Vast stores of secondary metabolites exist in plants, many of which possess biological activities related to human health. Phenolic acid derivatives are a class of valuable bioactive pharmaceuticals abundant in the widely used Chinese medicinal herb, Salvia miltiorrhiza. The biosynthetic pathway for phenolic acids differs in this species from that of other investigated plants. However, the molecular basis for this is unknown, with systematic analysis of the genes involved not yet performed. As the first step towards unraveling this complex biosynthetic pathway in S. miltiorrhiza, the current genome assembly was searched for putatively involved genes. Twenty-nine genes were revealed, 19 of which are described here for the first time. These include 15 genes predicted in the phenylpropanoid pathway; seven genes in the tyrosine-derived pathway; six genes encoding putative hydroxycinnamoyltransferases, and one CYP98A, namely CYP98A78. The promoter regions, gene structures and expression patterns of these genes were examined. Furthermore, conserved domains and phylogenetic relationships with homologous proteins in other species were revealed. Most of the key enzymes, including 4-coumarate: CoA ligase, 4-hydroxyphenylpyruvate reductase and hydroxycinnamoyltransferase, were found in multiple copies, each exhibiting different characteristics. Ten genes putatively involved in rosmarinic acid biosynthesis are also described. These findings provide a foundation for further analysis of this complex and diverse pathway, with potential to enhance the synthesis of water-soluble medicinal compounds in S. miltiorrhiza.
Keyword Phenolic acids
Phenolic acid biosynthesis
Rosmarinic acid
Medicinal compounds
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 81130069
2012BAI29B01
IRT1150
Institutional Status UQ
Additional Notes Published online ahead of print 04 Dec 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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