Isolation of high-lipid Tetraselmis suecica strains following repeated UV-C mutagenesis, FACS, and high-throughput growth selection

Lim, David K. Y., Schuhmann, Holger, Sharma, Kalpesh and Schenk, Peer M. (2014) Isolation of high-lipid Tetraselmis suecica strains following repeated UV-C mutagenesis, FACS, and high-throughput growth selection. Bioenergy Research, 8 2: 750-759. doi:10.1007/s12155-014-9553-2


Author Lim, David K. Y.
Schuhmann, Holger
Sharma, Kalpesh
Schenk, Peer M.
Title Isolation of high-lipid Tetraselmis suecica strains following repeated UV-C mutagenesis, FACS, and high-throughput growth selection
Formatted title
Isolation of high-lipid Tetraselmis suecica strains following repeated UV-C mutagenesis, FACS, and high-throughput growth selection
Journal name Bioenergy Research   Check publisher's open access policy
ISSN 1939-1242
1939-1234
Publication date 2014-11-14
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s12155-014-9553-2
Volume 8
Issue 2
Start page 750
End page 759
Total pages 10
Place of publication New York NY United States
Publisher Springer New York
Collection year 2015
Language eng
Formatted abstract
Mutagenesis and selection of microalgae can be used for accelerated breeding of elite strains, providing a significant advantage over genetic engineering as prior biochemical and genetic information is not required. Ultraviolet (UV)-C-induced mutagenesis combined with fluorescence-activated cell sorting (FACS) and microtiter plate reader cell density screening was used to produce Tetraselmis suecica strains with increased lipid contents without compromising on cell growth. After five rounds of mutation-selection, two dosages of UV-C (50 and >98 % lethality) yielded two improved strains (M5 and M24) that produced significantly more neutral lipids (increases of 114 and 123 %, respectively). This study highlights that repeated UV-C mutagenesis and high-throughput selection for cell growth can be a viable combined approach to improve lipid productivity in microalgae. These maybe used as elite strains for future breeding programs and as potential feedstock for biodiesel production.
Keyword Biodiesel
Breeding
Cell sorting
Lipid production
Microalgae
Mutation
Selection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 14 Nov 2014.

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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