Temporal changes in microbial community composition during culture enrichment experiments with Indonesian coals

Susilawati, Rita, Evans, Paul N., Esterle, Joan S., Robbins, Steven J., Tyson, Gene W., Golding, Suzanne D. and Mares, Tennille E. (2015) Temporal changes in microbial community composition during culture enrichment experiments with Indonesian coals. International Journal of Coal Geology, 137 66-76. doi:10.1016/j.coal.2014.10.015

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Author Susilawati, Rita
Evans, Paul N.
Esterle, Joan S.
Robbins, Steven J.
Tyson, Gene W.
Golding, Suzanne D.
Mares, Tennille E.
Title Temporal changes in microbial community composition during culture enrichment experiments with Indonesian coals
Journal name International Journal of Coal Geology   Check publisher's open access policy
ISSN 0166-5162
1872-7840
Publication date 2015-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.coal.2014.10.015
Open Access Status Not yet assessed
Volume 137
Start page 66
End page 76
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Temporal changes in microbial community structures during methanogenesis were investigated in cultures of South Sumatra Basin (SSB) coalbed methane (CBM) formation water (SSB5) grown on three coals of different rank (Burung sub bituminous Rv 039%, Mangus sub bituminous Rv 0.5%, Mangus anthracite Rv 22%). Methane production accelerated from day 6, peaked around day 17 and then levelled off around day 20. The initial bacterial community from the SSB formation water was predominantly Aceto bacterium, Acidaminobacter, Bacteroides and Pelobacter species, while the archaeal community consisted of Methanosaeta, Methanosarcina and Methanobacterium members. A general pattern was observed in all cultures with the three coals. Over time the bacterial members decreased in proportion whereas the archaeal component increased. The increase in the proportion of archaeal methanogens corresponded with an increase in methane production yield.
Formatted abstract
Temporal changes in microbial community structures during methanogenesis were investigated in cultures of South Sumatra Basin (SSB) coalbed methane (CBM) formation water (SSB5) grown on three coals of different rank (Burung sub bituminous Rv 0.39%, Mangus sub bituminous Rv 0.5%, Mangus anthracite Rv 2.2%). Methane production accelerated from day 6, peaked around day 17 and then levelled off around day 20. The initial bacterial community from the SSB formation water was predominantly Acetobacterium, Acidaminobacter, Bacteroides and Pelobacter species, while the archaeal community consisted of Methanosaeta, Methanosarcina and Methanobacterium members. A general pattern was observed in all cultures with the three coals. Over time the bacterial members decreased in proportion whereas the archaeal component increased. The increase in the proportion of archaeal methanogens corresponded with an increase in methane production yield.

Enrichment cultures produced similar communities when grown on coals from the same seam (Mangus sub bituminous and Mangus anthracite), rather than from different seams of similar type (Burung sub bituminous and Mangus sub bituminous). Methanosaeta was the dominant methanogen species in the sub bituminous Burung coal culture, but was a lesser proportion in cultures of both Mangus sub bituminous and anthracite coals where Methanosarcina species were a greater proportion. Interestingly, obligate hydrogenotrophic methanogens from the genera of Methanobacterium, which were present at low levels in culture enrichment of all coal substrates, increased in proportion only in the absence of coal in the no-coal control enrichment cultures. These results suggest that the low rank Burung sub bituminous coal favours methane production by the obligate acetoclastic Methanosaeta members while both Mangus coals also favour metabolically versatile Methanosarcina members, and the absence of coal favours hydrogenotrophic methanogens. Despite the similarity of communities grown on coals from the same seam, greater quantities of methane were generated from the lower rank coals when compared to higher rank coals.
Keyword Coal
Microbial community
Methanogenesis
Coalbed methane
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 7 Nov 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Earth Sciences Papers
Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 16 Dec 2014, 10:20:06 EST by System User on behalf of School of Earth Sciences