Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene

Menzies, Moira, Seim, Inge, Josh, Peter, Nagaraj, Shivashankar H., Lees, Michael, Walpole, Carina, Chopin, Lisa K., Colgrave, Michelle and Ingham, Aaron (2014) Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene. BMC Veterinary Research, 10 1: 211.1-211.9. doi:10.1186/s12917-014-0211-x

Author Menzies, Moira
Seim, Inge
Josh, Peter
Nagaraj, Shivashankar H.
Lees, Michael
Walpole, Carina
Chopin, Lisa K.
Colgrave, Michelle
Ingham, Aaron
Title Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene
Journal name BMC Veterinary Research   Check publisher's open access policy
ISSN 1746-6148
Publication date 2014-09-06
Sub-type Article (original research)
DOI 10.1186/s12917-014-0211-x
Open Access Status DOI
Volume 10
Issue 1
Start page 211.1
End page 211.9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Formatted abstract
Background: The ghrelin axis is involved in the regulation of metabolism, energy balance, and the immune, cardiovascular and reproductive systems. The manipulation of this axis has potential for improving economically valuable traits in production animals, and polymorphisms in the ghrelin (GHRL) and ghrelin receptor (GHSR) genes have been associated with growth and carcass traits. Here we investigate the structure and expression of the ghrelin gene (GHRL) in sheep, Ovis aries.

Results: We identify two ghrelin mRNA isoforms, which we have designated Δex2 preproghrelin and Δex2,3 preproghrelin. Expression of Δex2,3 preproghrelin is likely to be restricted to ruminants, and would encode truncated ghrelin and a novel C-terminal peptide. Both Δex2 preproghrelin and canonical preproghrelin mRNA isoforms were expressed in a range of tissues. Expression of the Δex2,3 preproghrelin isoform, however, was restricted to white blood cells (WBC; where the wild-type preproghrelin isoform is not co-expressed), and gastrointestinal tissues. Expression of Δex2 preproghrelin and Δex2,3 preproghrelin mRNA was elevated in white blood cells in response to parasitic worm (helminth) infection in genetically susceptible sheep, but not in resistant sheep.

Conclusions: The restricted expression of the novel preproghrelin variants and their distinct WBC expression pattern during parasite infection may indicate a novel link between the ghrelin axis and metabolic and immune function in ruminants.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
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