Poly(lactide-co-glycolide) ultrasonographic microbubbles carrying sudan black for preoperative and intraoperative localization of lymph nodes

Niu, Chengcheng, Wang, Zhigang, Zuo, Guoqing, Krupka, Tianyi M., Ran, Haitao, Zhang, Ping, Li, Pan, Chen, Yu, Chen, Hangrong and Zheng, Yuanyi (2012) Poly(lactide-co-glycolide) ultrasonographic microbubbles carrying sudan black for preoperative and intraoperative localization of lymph nodes. Clinical Breast Cancer, 12 3: 199-206. doi:10.1016/j.clbc.2012.01.005


Author Niu, Chengcheng
Wang, Zhigang
Zuo, Guoqing
Krupka, Tianyi M.
Ran, Haitao
Zhang, Ping
Li, Pan
Chen, Yu
Chen, Hangrong
Zheng, Yuanyi
Title Poly(lactide-co-glycolide) ultrasonographic microbubbles carrying sudan black for preoperative and intraoperative localization of lymph nodes
Journal name Clinical Breast Cancer   Check publisher's open access policy
ISSN 1526-8209
1938-0666
Publication date 2012-06-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.clbc.2012.01.005
Open Access Status Not yet assessed
Volume 12
Issue 3
Start page 199
End page 206
Total pages 8
Place of publication New York, NY, United States
Publisher Elsevier
Language eng
Abstract Lymph node (LN) examination plays a critical role in the staging and treatment of several kinds of cancer such as lesions of the breast. However current strategies have limitations. This study aimed to develop a novel imaging agent, a polymeric ultrasonographic contrast agent carrying Sudan black (SB), for ultrasonographic imaging of the regional LNs before surgery and to directly localize the LNs during surgery. The poly(lactide-co-glycolide) (PLGA) ultrasonographic microbubbles carrying Sudan black B (SB) (SB-PLGA microbubbles) were prepared by the double emulsion method. The SB-PLGA microbubbles had a diameter of 1.5 ± 0.5 μm and the SB encapsulation efficiency was (86.2 ± 1.56%). Results from MTT assays suggested that these bubbles have little cytotoxicity to mouse macrophages after incubation. Confocal laser scanning microscopy showed that the PLGA microbubbles carrying the fluorescent dye rhodamine 6G were taken up by macrophages after 2-hour incubation. In addition, these SB-PLGA microbubbles were able to enhance ultrasonographic contrast of 12 popliteal LNs of 6 rabbits. Furthermore, the LNs were easily identifiable by the naked eye during surgery because of the blue color of the SB-PLGA microbubbles inside the LNs. By cryosectioning and hematoxylin and eosin (H&E) staining of LN tissue, our results showed that these SB-PLGA microbubbles were internalized inside the macrophages of the LNs. To conclude, the SB-PLGA microbubbles could be a suitable imaging agent for preoperative and intraoperative localization of LNs as well as for a preoperative ultrasonographically guided core needle biopsy of suspicious sentinel lymph nodes (SLNs) in cancer patients, hence enhancing treatment outcome.
Keyword Lymph nodes
PLGA microbubbles
Sudan black
Ultrasonography
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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