The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

He, Qianjun, Zhang, Jiamin, Shi, Jianlin, Zhu, Ziyan, Zhang, Linxia, Bu, Wenbo, Guo, Limin and chen, Yu (2010) The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses. Biomaterials, 31 6: 1085-1092. doi:10.1016/j.biomaterials.2009.10.046


Author He, Qianjun
Zhang, Jiamin
Shi, Jianlin
Zhu, Ziyan
Zhang, Linxia
Bu, Wenbo
Guo, Limin
chen, Yu
Title The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2010-02-01
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2009.10.046
Open Access Status Not yet assessed
Volume 31
Issue 6
Start page 1085
End page 1092
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Highly ordered MCM-41-type mesoporous silica nanoparticles (MSNs) with particle sizes of 150 ± 20 nm were prepared and PEGylated by covalently grafting PEGxk chains of different molecular weights (x = 4, 6, 10, 20) and chain densities (0.05 wt%-3.75 wt%) on the outer surface. The influence of molecular weights and chain densities of PEGxk on the nonspecific binding of PEGylated MSNs to human serum protein (HSA) was investigated. The results revealed that the optimal molecular weights should be not less than 10 k, and the corresponding optimal chain densities for PEG10k-MSNs and PEG20k-MSNs were 0.75 wt% and 0.075 wt%, respectively, and the resultant minimum HSA adsorbance (2.5%) on PEGxk-MSNs was far less than that on MSNs (18.7%) without PEGylation. Under the optimal conditions for the minimum HSA adsorbance, the phagocytosis of human THP-1 monocytic leukemia cell line-derived macrophages (THP-1 macrophages) and the hemolysis of human red blood cells (HRBCs) were investigated with MSNs and PEGylated MSNs. A minimum THP-1 phagocytosis percentage (0.1%) and a very low HRBCs hemolysis percentage (0.9%) of PEG10k-MSNs were obtained, which were much lower than those (8.6% and 14.2%, respectively) of MSNs.
Keyword Hemolysis
Mesoporous silica
Nanoparticle
PEG
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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