The red nucleus and the rubrospinal projection in the mouse

Liang, Huazheng, Paxinos, George and Watson, Charles (2012) The red nucleus and the rubrospinal projection in the mouse. Brain Structure and Function, 217 2: 221-232. doi:10.1007/s00429-011-0348-3

Author Liang, Huazheng
Paxinos, George
Watson, Charles
Title The red nucleus and the rubrospinal projection in the mouse
Journal name Brain Structure and Function   Check publisher's open access policy
ISSN 1863-2653
Publication date 2012-04-01
Sub-type Article (original research)
DOI 10.1007/s00429-011-0348-3
Open Access Status Not Open Access
Volume 217
Issue 2
Start page 221
End page 232
Total pages 12
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract We studied the organization and spinal projection of the mouse red nucleus with a range of techniques (Nissl stain, immunofluorescence, retrograde tracer injections into the spinal cord, anterograde tracer injections into the red nucleus, and in situ hybridization) and counted the number of neurons in the red nucleus (3,200.9 ± 230.8). We found that the rubrospinal neurons were mainly located in the parvicellular region of the red nucleus, more lateral in the rostral part and more medial in the caudal part. Labeled neurons were least common in the rostral and caudal most parts of the red nucleus. Neurons projecting to the cervical cord were predominantly dorsomedially placed and neurons projecting to the lumbar cord were predominantly ventrolaterally placed. Immunofluorescence staining with SMI-32 antibody showed that ∼60% of SMI-32- positive neurons were cervical cord-projecting neurons and 24% were lumbar cord-projecting neurons. SMI-32-positive neurons were mainly located in the caudomedial part of the red nucleus. A study of vGluT2 expression showed that the number and location of glutamatergic neurons matched with those of the rubrospinal neurons. In the anterograde tracing experiments, rubrospinal fibers travelled in the dorsal portion of the lateral funiculus, between the lateral spinal nucleus and the calretinin-positive fibers of the lateral funiculus. Rubrospinal fibers terminated in contralateral laminae 5, 6, and the dorsal part of lamina 7 at all spinal cord levels. A few fibers could be seen next to the neurons in the dorsolateral part of lamina 9 at levels of C8- T1 (hand motor neurons) and L5-L6 (foot motor neurons), which is consistent with a view that rubrospinal fibers may play a role in distal limb movement in rodents.
Keyword C1QL2
Prosomere 1
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 28 Oct 2014, 02:33:45 EST by Sylvie Pichelin on behalf of Queensland Brain Institute