Detecting exosomes specifically: a multiplexed device based on alternating current electrohydrodynamic induced nanoshearing

Vaidyanathan, Ramanathan, Naghibosadat, Maedeh, Rauf, Sakandar, Korbie, Darren, Carrascosa, Laura G., Shiddiky, Muhammad J. A. and Trau, Matt (2014) Detecting exosomes specifically: a multiplexed device based on alternating current electrohydrodynamic induced nanoshearing. Analytical Chemistry, 86 22: 11125-11132. doi:10.1021/ac502082b

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Author Vaidyanathan, Ramanathan
Naghibosadat, Maedeh
Rauf, Sakandar
Korbie, Darren
Carrascosa, Laura G.
Shiddiky, Muhammad J. A.
Trau, Matt
Title Detecting exosomes specifically: a multiplexed device based on alternating current electrohydrodynamic induced nanoshearing
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 1520-6882
0003-2700
Publication date 2014-10-17
Year available 2014
Sub-type Article (original research)
DOI 10.1021/ac502082b
Open Access Status Not yet assessed
Volume 86
Issue 22
Start page 11125
End page 11132
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Exosomes show promise as non-invasive biomarkers for cancers, but their effective capture and specific detection is a significant challenge. Herein, we report a multiplexed microfluidic device for highly specific capture and detection of multiple exosome targets using a tuneable alternating current electrohydrodynamic (ac-EHD) methodology - referred to as nanoshearing. In our system, electrical body forces generated by ac-EHD act within nanometers of an electrode surface (i.e., within the electrical layer) to generate nanoscaled fluid flow which enhances the specificity of capture and also reduce nonspecific adsorption of weakly bound molecules from the electrode surface. This approach demonstrates the analysis of exosomes derived from cells expressing human epidermal growth factor receptor 2 (HER2) and prostate specific antigen (PSA), and exhibits a 5-fold detection enhancement compared to hydrodynamic flow based assays. The device was also sensitive enough to detect approximately 2750 exosomes/µL (n = 3) and also capable of specifically isolating exosomes from breast cancer patient samples. We believe this approach can potentially find its relevance as a simple and rapid quantification tool to analyze exosome targets in biological applications.
Keyword AC electrohydrodynamics
Exosomes
Multiplex detection
Microfluidic devices
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Publication Date (Web): 17 Oct 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 22 Oct 2014, 00:11:16 EST by Ramanathan Pudhukode Vaidyanathan on behalf of Aust Institute for Bioengineering & Nanotechnology