Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense

Fraser-Smith, S., Czislowski, E., Meldrum, R. A., Zander, M., O'Neill, W., Balali, G. R. and Aitken, E. A. B. (2014) Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense. Plant Pathology, 63 5: 1044-1052. doi:10.1111/ppa.12184


Author Fraser-Smith, S.
Czislowski, E.
Meldrum, R. A.
Zander, M.
O'Neill, W.
Balali, G. R.
Aitken, E. A. B.
Title Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense
Journal name Plant Pathology   Check publisher's open access policy
ISSN 0032-0862
1365-3059
Publication date 2014-10-01
Year available 2014
Sub-type Article (original research)
DOI 10.1111/ppa.12184
Open Access Status Not yet assessed
Volume 63
Issue 5
Start page 1044
End page 1052
Total pages 9
Place of publication HOBOKEN
Publisher Blackwell Publishing Ltd
Language eng
Subject 1311 Genetics
1108 Medical Microbiology
1110 Nursing
1102 Cardiovascular Medicine and Haematology
Abstract Fusarium oxysporum f. sp. cubense (Foc), causal agent of fusarium wilt of banana, is among the most destructive pathogens of banana and plantain. The development of a molecular diagnostic capable of reliably distinguishing between the various races of the pathogen is of key importance to disease management. However, attempts to distinguish isolates using the standard molecular loci typically used for fungal phylogenetics have been complicated by a poor correlation between phylogeny and pathogenicity. Among the available alternative loci are several putative effector genes, known as SIX genes, which have been successfully used to differentiate the three races of F. oxysporum f. sp. lycopersici. In this study, an international collection of Foc isolates was screened for the presence of the putative effector SIX8. Using a PCR and sequencing approach, variation in Foc-SIX8 was identified which allowed race 4 to be differentiated from race 1 and 2 isolates, and tropical and subtropical race 4 isolates to be distinguished from one another.
Keyword Avirulence
Effector
Fusarium oxysporum f. sp. cubense
Molecular diagnostic
Pathogenicity
SIX
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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