Isolation and characterisation of 18 polymorphic microsatellite loci for black marlin (Istiompax indica) and their utility for Pacific billfish species

Williams, Samuel M., Pepperell, Julian G., Corley, Sean W. and Ovenden, Jennifer R. (2015) Isolation and characterisation of 18 polymorphic microsatellite loci for black marlin (Istiompax indica) and their utility for Pacific billfish species. Fisheries Research, 166 29-32. doi:10.1016/j.fishres.2014.07.005


 
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Author Williams, Samuel M.
Pepperell, Julian G.
Corley, Sean W.
Ovenden, Jennifer R.
Title Isolation and characterisation of 18 polymorphic microsatellite loci for black marlin (Istiompax indica) and their utility for Pacific billfish species
Formatted title
Isolation and characterisation of 18 polymorphic microsatellite loci for black marlin (Istiompax indica) and their utility for Pacific billfish species
Journal name Fisheries Research   Check publisher's open access policy
ISSN 0165-7836
1872-6763
Publication date 2015-06-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.fishres.2014.07.005
Open Access Status Not yet assessed
Volume 166
Start page 29
End page 32
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
We present the isolation and characterisation of 18 polymorphic microsatellite loci with perfect 3 and 4-bp repeats for the black marlin Istiompax indica using Ion Torrent next-generation sequencing techniques. Usefulness of these markers for genetic studies was assessed by screening 16 specimens collected throughout the Indo-Pacific basin for this broadly distributed and exploited species. A total of 30 black marlin from the West Coral Sea were genotyped producing between 2 and 20 alleles per locus (mean 5.27) with expected heterozygosity (HE) ranging from 0.095 to 0.843. The genotypic distribution of all loci conformed to Hardy-Weinberg expectations with no evidence of null alleles. Evaluations of cross-species amplification of these markers on five additional Pacific billfish species indicates the utility of these markers for genetic studies of other Istiophorids.
Keyword Billfish
Fisheries management
Population genetics
Next-generation sequencing
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
School of Veterinary Science Publications
 
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