Functional characterization of SjB10, an intracellular serpin from Schistosoma japonicum

Molehin, Adebayo J., Gobert, Geoffrey N., Driguez, Patrick and McManus, Donald P. (2014) Functional characterization of SjB10, an intracellular serpin from Schistosoma japonicum. Parasitology, 141 13: 1746-1760. doi:10.1017/S0031182014001061


Author Molehin, Adebayo J.
Gobert, Geoffrey N.
Driguez, Patrick
McManus, Donald P.
Title Functional characterization of SjB10, an intracellular serpin from Schistosoma japonicum
Formatted title
Functional characterization of SjB10, an intracellular serpin from Schistosoma japonicum
Journal name Parasitology   Check publisher's open access policy
ISSN 0031-1820
1469-8161
Publication date 2014-11-01
Year available 2014
Sub-type Article (original research)
DOI 10.1017/S0031182014001061
Volume 141
Issue 13
Start page 1746
End page 1760
Total pages 15
Place of publication Cambridge, United Kingdom
Publisher Cambridge University Press
Collection year 2015
Language eng
Formatted abstract
Serine protease inhibitors (serpin) play essential roles in many organisms. Mammalian serpins regulate the blood coagulation, fibrinolysis, inflammation and complement activation pathways. In parasitic helminths, serpins are less well characterized, but may also be involved in evasion of the host immune response. In this study, a Schistosoma japonicum serpin (SjB10), containing a 1212 bp open reading frame (ORF), was cloned, expressed and functionally characterized. Sequence analysis, comparative modelling and structural-based alignment revealed that SjB10 contains the essential structural motifs and consensus secondary structures of inhibitory serpins. Transcriptional profiling demonstrated that SjB10 is expressed in adult males, schistosomula and eggs but particularly in the cercariae, suggesting a possible role in cercarial penetration of mammalian host skin. Recombinant SjB10 (rSjB10) inhibited pancreatic elastase (PE) in a dose-dependent manner. rSjB10 was recognized strongly by experimentally infected rat sera indicating that native SjB10 is released into host tissue and induces an immune response. By immunochemistry, SjB10 localized in the S. japonicum adult foregut and extra-embryonic layer of the egg. This study provides a comprehensive demonstration of sequence and structural-based analysis of a functional S. japonicum serpin. Furthermore, our findings suggest that SjB10 may be associated with important functional roles in S. japonicum particularly in host-parasite interactions.
Keyword Schistosoma japonicum
Serine protease inhibitors
SjB10
Structure-to-function analysis
Functional characterization
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Public Health Publications
 
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