Survival in sterile soil and atrazine degradation of Pseudomonas sp. strain ADP immobilized on zeolite

Stelting, Scott A., Burns, Richard G., Sunna, Anwar and Bunt, Craig R. (2014) Survival in sterile soil and atrazine degradation of Pseudomonas sp. strain ADP immobilized on zeolite. Bioremediation Journal, 18 4: 309-316. doi:10.1080/10889868.2014.938723


Author Stelting, Scott A.
Burns, Richard G.
Sunna, Anwar
Bunt, Craig R.
Title Survival in sterile soil and atrazine degradation of Pseudomonas sp. strain ADP immobilized on zeolite
Formatted title
Survival in sterile soil and atrazine degradation of Pseudomonas sp. strain ADP immobilized on zeolite
Journal name Bioremediation Journal   Check publisher's open access policy
ISSN 1088-9868
1547-6529
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1080/10889868.2014.938723
Volume 18
Issue 4
Start page 309
End page 316
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Collection year 2015
Language eng
Formatted abstract
In laboratory settings, the ability of bacteria and fungi to degrade many environmental contaminants is well proven. However, the potential of microbial inoculants in soil remediation has not often been realized because catabolically competent strains rarely survive and proliferate in soil, and even if they do, they usually fail to express their desired catabolic potential. One method to address the survival problem is formulating the microorganisms with physical and chemical support systems. This study investigates the survival of Pseudomonas sp. strain ADP in sterile soil and its retention of atrazine-degrading functionality. Assessment was conducted with free and zeolite-immobilized bacteria incorporated into the soil. Pseudomonas sp. strain ADP remained viable for at least 10 weeks when stored at 15°C in sterile soil. Cell numbers increased for both free and zeolite-immobilized bacteria during this period, except for free cells when grown in Miller's Luria-Bertani medium, which exhibited constant cell numbers over the 10 weeks. Only the zeolite-immobilized cell retained full functionality to degrade atrazine after 10 weeks in sterile soil regardless of the medium used to culture Pseudomonas sp. strain ADP. Functionality was diminished in free-cell inoculations except when using an improved culture medium. Survival of zeolite-immobilized Pseudomonas sp. strain ADP separated from the soil matrix after 10 weeks’ incubation was significantly (p < .05) greater than in soil inoculated with free cells or in the soil fraction inoculated by release from zeolite-immobilized Pseudomonas sp. strain ADP.
Keyword Zeolite
Immobilized cells
Formulation
Bioremediation
Bacterial survival
Stabilization
Growth-promoting bacteria
White-rot fungus
Environmental applications
Microbial cells
Microorganisms
Bioaugmentation
Biodegradation
Encapsulation
Fluorescens
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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