The effect of fibre source on the numbers of some fibre degrading bacteria of Arabian camel’s (Camelus dromedarius) foregut origin

Samsudin, Anjas Asmara, Wright, André-Denis and Al Jassim, Rafat (2014) The effect of fibre source on the numbers of some fibre degrading bacteria of Arabian camel’s (Camelus dromedarius) foregut origin. Tropical Animal Health and Production, 46 7: 1161-1166. doi:10.1007/s11250-014-0621-6


Author Samsudin, Anjas Asmara
Wright, André-Denis
Al Jassim, Rafat
Title The effect of fibre source on the numbers of some fibre degrading bacteria of Arabian camel’s (Camelus dromedarius) foregut origin
Formatted title
The effect of fibre source on the numbers of some fibre degrading bacteria of Arabian camel’s (Camelus dromedarius) foregut origin
Journal name Tropical Animal Health and Production   Check publisher's open access policy
ISSN 0049-4747
1573-7438
Publication date 2014-01-01
Sub-type Article (original research)
DOI 10.1007/s11250-014-0621-6
Open Access Status
Volume 46
Issue 7
Start page 1161
End page 1166
Total pages 6
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Abstract The total bacterial community of Fibrobacter succinogenes and Ruminococcus flavefaciens in fibre-enriched culture of the foregut contents of 12 adult feral camels (Camelus dromedaries) fed on native vegetation in Australia was investigated using quantitative PCR. Foregut contents were collected postmortem, pooled and filtered before divided into two fractions. One fraction was used for extraction of DNA, while the other fraction was inoculated straight away into BM 10 contained filter paper (FP), cotton thread (CT) or neutral detergent fibre (NDF) as the sole carbohydrate sources in Hungate tubes. The tubes were incubated anaerobically at 39 °C for 1 week. After a near complete degradation of the FP and CT and extensive turbidity in the NDF, media subculturing was carried out into fresh media tubes. This was repeated twice before genomic DNA was extracted and used for quantification of bacteria. Using an absolute quantification method, the numbers of cells in 1 ml of each sample ranged from 4.07 × 106 to 2.73 × 109 for total bacteria, 1.34 × 103 to 2.17 × 105 for F. succinogenes and 5.78 × 101 to 3.53 × 104 for R. flavefaciens. The mean cell number of F. succinogenes was highest in the FP enrichment medium at approximately 107-fold, whereas for the R. flavefaciens targeted primer, the NDF enrichment media had the highest mean cell number at approximately 4-fold when compared to the rumen content. The data presented here provide evidence of fibre type preference by the two main fibre-degrading bacteria and would help us understand the interaction between fibre type and fibre-degrading microorganisms, which has ramification on camel nutrition at different seasons and environments.
Keyword Arabian camel
Fibre-degrading bacteria
Native vegetation
Real-time PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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Created: Thu, 02 Oct 2014, 23:43:27 EST by Dr Rafat Al Jassim on behalf of School of Agriculture and Food Sciences