Defining a proinflammatory neutrophil phenotype in response to Schistosome eggs

Chuah, Candy, Jones, Malcolm K., Burke, Melissa L., McManus, Donald P., Owen, Helen C. and Gobert, Geoffrey N. (2014) Defining a proinflammatory neutrophil phenotype in response to Schistosome eggs. Cellular Microbiology, 16 11: 1666-1677. doi:10.1111/cmi.12316


Author Chuah, Candy
Jones, Malcolm K.
Burke, Melissa L.
McManus, Donald P.
Owen, Helen C.
Gobert, Geoffrey N.
Title Defining a proinflammatory neutrophil phenotype in response to Schistosome eggs
Journal name Cellular Microbiology   Check publisher's open access policy
ISSN 1462-5814
1462-5822
Publication date 2014-06-25
Year available 2014
Sub-type Article (original research)
DOI 10.1111/cmi.12316
Volume 16
Issue 11
Start page 1666
End page 1677
Total pages 12
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Neutrophils contribute to the pathological processes of a number of inflammatory disorders, including rheumatoid arthritis, sepsis and cystic fibrosis. Neutrophils also play prominent roles in schistosomiasis japonica liver fibrosis, being central mediators of inflammation following granuloma formation. In this study, we investigated the interaction between Schistosoma japonicum eggs and neutrophils, and the effect of eggs on the inflammatory phenotype of neutrophils. Our results showed significant upregulated expression of pro-inflammatory cytokines (IL-1α, IL-1β and IL-8) and chemokines (CCL3, CCL4 and CXCL2) in neutrophils after 4 h in vitro stimulation with S. japonicum eggs. Furthermore, mitochondrial DNA was released by stimulated neutrophils, and induced the production of matrix metalloproteinase 9 (MMP-9), a protease involved in inflammation and associated tissue destruction. We also found that intact live eggs and isolated soluble egg antigen (SEA) triggered the release of neutrophil extracellular traps (NETs), but, unlike those reported in bacterial or fungal infection, NETs did not kill schistosome eggs in vitro. Together these show that S. japonicum eggs can induce the inflammatory phenotype of neutrophils, and further our understanding of the host-parasite interplay that takes place within the in vivo microenvironment of schistosome-induced granuloma. These findings represent novel findings in a metazoan parasite, and confirm characteristics of NETs that have until now, only been observed in response to protozoan pathogens.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
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Created: Thu, 02 Oct 2014, 22:46:27 EST by Dr Helen Owen on behalf of School of Veterinary Science