Herpesvirus mutagenesis facilitated by infectious bacterial artificial chromosomes (iBACs)

Robinson, Karl E. and Mahony, Timothy J. (2015). Herpesvirus mutagenesis facilitated by infectious bacterial artificial chromosomes (iBACs). In Kumaran Narayanan (Ed.), Bacterial artificial chromosomes (pp. 181-197) New York, NY, United States: Springer Science+Business Media. doi:10.1007/978-1-4939-1652-8_8

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Author Robinson, Karl E.
Mahony, Timothy J.
Title of chapter Herpesvirus mutagenesis facilitated by infectious bacterial artificial chromosomes (iBACs)
Title of book Bacterial artificial chromosomes
Place of Publication New York, NY, United States
Publisher Springer Science+Business Media
Publication Year 2015
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-1652-8_8
Open Access Status Not yet assessed
Year available 2014
Series Methods in Molecular Biology
ISBN 9781493916511
ISSN 1064-3745
Editor Kumaran Narayanan
Volume number 1227
Chapter number 8
Start page 181
End page 197
Total pages 17
Total chapters 18
Language eng
Abstract/Summary A critical factor in the study of herpesviruses, their genes and gene functions is the capacity to derive mutants that harbor deletions, truncations, or insertions within the genetic elements of interest. Once constructed the impact of the introduced mutation on the phenotypic properties of the rescued virus can be determined in either in vitro or in vivo systems. However, the construction of such mutants by traditional virological mutagenesis techniques can be a difficult and laborious undertaking. The maintenance of a viral genome as an infectious bacterial artificial chromosome (iBAC), however, endows the capacity to manipulate the viral genome for mutagenesis studies with relative ease. Here, the construction and characterization of two gene deletion mutants of an alphaherpesvirus maintained as iBAC in combination with an inducible homologous recombination system in Escherichia coli is detailed. The methodology is generally applicable to any iBAC and is demonstrated to be a highly efficient and informative approach for mutant virus construction.
Keyword Herpesvirus
Bacterial artificial chromosomes
Homologous recombination
Gene deletion
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Tue, 30 Sep 2014, 20:45:24 EST by Karl Robinson on behalf of Centre for Crop Science