Further characterisation of the immune response of the koala

Wilkinson, R., Kotlarski, I. and Barton, M. (1994) Further characterisation of the immune response of the koala. Veterinary Immunology and Immunopathology, 40 4: 325-339. doi:10.1016/0165-2427(94)90043-4

Author Wilkinson, R.
Kotlarski, I.
Barton, M.
Title Further characterisation of the immune response of the koala
Journal name Veterinary Immunology and Immunopathology   Check publisher's open access policy
ISSN 0165-2427
Publication date 1994-04-01
Sub-type Article (original research)
DOI 10.1016/0165-2427(94)90043-4
Open Access Status Not Open Access
Volume 40
Issue 4
Start page 325
End page 339
Total pages 15
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Sensitive enzyme immunoassays (EIA) were developed to monitor antibody (Ab) production in the koala, in response to both soluble and particulate antigens (Ag). When compared with a eutherian mammal, the rabbit, both the dynamics and kinetics of Ab production in the koala were found to be severely retarded. In vitro, Ag specific lymphocyte proliferative responses were demonstrated for the first time in this animal by sensitising koalas in vivo with Bacillus Calmet-Guerin (BCG), with the level and timing of this cell mediated immune (CMI) response comparable with those seen in non-metatherian mammals. Levels of circulating B lymphocytes were examined in an attempt to clarify the retarded humoral responses to foreign Ags. In addition, peripheral mononuclear cells (PMC) from koalas, were examined for their reactivity to a range of monoclonal Abs and lectins in an attempt to characterise these cells further. The lectins examined, demonstrated an all or none reactivity with koala lymphocytes and were therefore considered unsuitable as markers for identifying lymphoid subsets in this animal. A monoclonal Ab directed at class II MHC Ags in the mouse, demonstrated cross reactivity with a high percentage of all koala monocytes tested. Using this Ab to probe CMI responses in vitro, it is concluded that immune interactions required for such responses in the koala parallel those seen in other mammals.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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