The intimin-like protein FdeC is regulated by H-NS and temperature in Enterohaemorrhagic Escherichia coli

Easton, Donna M., Allsopp, Luke P., Phan, Minh-Duy, Gomes Moriel, Danilo, Goh, Guan Kai, Beatson, Scott A., Mahony, Timothy J., Cobbold, Rowland N. and Schembri, Mark A. (2014) The intimin-like protein FdeC is regulated by H-NS and temperature in Enterohaemorrhagic Escherichia coli. Applied and Environmental Microbiology, 80 23: 7337-7347. doi:10.1128/AEM.02114-14

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Author Easton, Donna M.
Allsopp, Luke P.
Phan, Minh-Duy
Gomes Moriel, Danilo
Goh, Guan Kai
Beatson, Scott A.
Mahony, Timothy J.
Cobbold, Rowland N.
Schembri, Mark A.
Title The intimin-like protein FdeC is regulated by H-NS and temperature in Enterohaemorrhagic Escherichia coli
Formatted title
The intimin-like protein FdeC is regulated by H-NS and temperature in Enterohaemorrhagic Escherichia coli
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2014-09-19
Year available 2014
Sub-type Article (original research)
DOI 10.1128/AEM.02114-14
Open Access Status File (Publisher version)
Volume 80
Issue 23
Start page 7337
End page 7347
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 1305 Biotechnology
1106 Food Science
2402 Applied Microbiology and Biotechnology
2303 Ecology
Abstract Enterohemorrhagic Escherichia coli (EHEC) is a Shiga-toxigenic pathogen capable of inducing severe forms of enteritis (e.g., hemorrhagic colitis) and extraintestinal sequelae (e.g., hemolytic-uremic syndrome). The molecular basis of colonization of human and animal hosts by EHEC is not yet completely understood, and an improved understanding of EHEC mucosal adherence may lead to the development of interventions that could disrupt host colonization. FdeC, also referred to by its IHE3034 locus tag ECOK1_0290, is an intimin-like protein that was recently shown to contribute to kidney colonization in a mouse urinary tract infection model. The expression of FdeC is tightly regulated in vitro, and FdeC shows promise as a vaccine candidate against extraintestinal E. coli strains. In this study, we characterized the prevalence, regulation, and function of fdeC in EHEC. We showed that the fdeC gene is conserved in both O157 and non-O157 EHEC and encodes a protein that is expressed at the cell surface and promotes biofilm formation under continuous-flow conditions in a recombinant E. coli strain background. We also identified culture conditions under which FdeC is expressed and showed that minor alterations of these conditions, such as changes in temperature, can significantly alter the level of FdeC expression. Additionally, we demonstrated that the transcription of the fdeC gene is repressed by the global regulator H-NS. Taken together, our data suggest a role for FdeC in EHEC when it grows at temperatures above 37 degrees C, a condition relevant to its specialized niche at the rectoanal junctions of cattle.
Keyword Biotechnology & Applied Microbiology
Microbiology
Biotechnology & Applied Microbiology
Microbiology
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 2008004328
DP1097032
FT100100662
Institutional Status UQ
Additional Notes Published online: 19 September 2014

 
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Created: Fri, 26 Sep 2014, 20:38:01 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences