Differential Endocytosis of Cd4 in Lymphocytic and Nonlymphocytic Cells

Pelchenmatthews, A, Armes, JE, Griffiths, G and Marsh, M (1991) Differential Endocytosis of Cd4 in Lymphocytic and Nonlymphocytic Cells. Journal of Experimental Medicine, 173 3: 575-587. doi:10.1084/jem.173.3.575

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Author Pelchenmatthews, A
Armes, JE
Griffiths, G
Marsh, M
Title Differential Endocytosis of Cd4 in Lymphocytic and Nonlymphocytic Cells
Journal name Journal of Experimental Medicine   Check publisher's open access policy
ISSN 0022-1007
Publication date 1991-03-01
Year available 1991
Sub-type Article (original research)
DOI 10.1084/jem.173.3.575
Open Access Status File (Publisher version)
Volume 173
Issue 3
Start page 575
End page 587
Total pages 13
Place of publication NEW YORK
Language eng
Abstract The endocytosis of the T cell differentiation antigen CD4 has been investigated in CD4-transfected HeLa cells, the promyelocytic HL-60 cell line, and in a number of leukemia- or lymphoma-derived T cells lines. CD4 internalization was followed using radioiodinated antibodies in an acid-elution endocytosis assay, or by covalently modifying cell surface proteins with biotin and analyzing CD4 distributions by immunoprecipitation; both approaches gave equivalent results. The assays demonstrated that in transfected HeLa cells and in HL-60 cells CD4 was constitutively internalized and recycled in the absence of ligand. Immunogold labeling and electron microscopy demonstrated that CD4 enters cells through coated pits.
Keyword Human Immunodeficiency Virus
Receptor-Mediated Endocytosis
Semliki Forest Virus
Protein Kinase-C
Phorbol Esters
Cytoplasmic Domain
Pinocytosed Fluid
Negative Signal
Hiv Infection
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
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