Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish

Hogan B.M., Layton J.E., Pyati U.J., Nutt S.L., Hayman J.W., Varma S., Heath J.K., Kimelman D. and Lieschke G.J. (2006) Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish. Current Biology, 16 5: 506-511. doi:10.1016/j.cub.2006.01.047

Author Hogan B.M.
Layton J.E.
Pyati U.J.
Nutt S.L.
Hayman J.W.
Varma S.
Heath J.K.
Kimelman D.
Lieschke G.J.
Title Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish
Journal name Current Biology   Check publisher's open access policy
ISSN 0960-9822
Publication date 2006-03-07
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.cub.2006.01.047
Open Access Status Not yet assessed
Volume 16
Issue 5
Start page 506
End page 511
Total pages 6
Publisher CELL PRESS
Language eng
Subject 1100 Agricultural and Biological Sciences
Abstract In the zebrafish embryo, primitive hematopoiesis initiates in two spatially distinct regions. Rostrally, the cells of the anterior lateral plate mesoderm (ALPM) give rise exclusively to cells of the myeloid lineage in a pu.1-dependent manner [1-5]. Caudally, in the posterior lateral plate mesoderm (PLPM), the expression of gata1 defines a precursor pool that gives rise predominantly to the embryonic erythrocytes [6]. The transcription factor scl acts upstream of both gata1 and pu.1 in these precursor pools, activating a series of conserved transcription factors that cell-autonomously specify either myeloid or erythroid fates [1, 4, 7, 8]. However, the mechanisms underlying the spatial separation of the hematopoietic precursor pools and the induction of differential gene expression within these pools are not well understood. We show here that the Bmp receptor lost-a-fin/alk8 is required for rostral pu.1 expression and myelopoiesis, identifying an early genetic event that distinguishes between the induction of anterior and posterior hematopoiesis. Introducing a constitutively active version of the Alk8 receptor led to increased pu.1 expression, but the role of alk8 was independent of the scl-dependent cell-fate pathway. Furthermore, the role of Alk8 in myelopoiesis was genetically separable from its earlier role in dorsal-ventral embryonic patterning.
Keyword Biochemistry & Molecular Biology
Cell Biology
Biochemistry & Molecular Biology
Cell Biology
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID P40 RR12546
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
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