Expanding our view of genomic diversity in Candidatus Accumulibacter clades

Skennerton, Connor T., Barr, Jeremy J., Slater, Frances R., Bond, Philip L. and Tyson, Gene W. (2014) Expanding our view of genomic diversity in Candidatus Accumulibacter clades. Environmental Microbiology, 17 5: 1574-1585. doi:10.1111/1462-2920.12582

Author Skennerton, Connor T.
Barr, Jeremy J.
Slater, Frances R.
Bond, Philip L.
Tyson, Gene W.
Title Expanding our view of genomic diversity in Candidatus Accumulibacter clades
Formatted title
Expanding our view of genomic diversity in Candidatus Accumulibacter clades
Journal name Environmental Microbiology   Check publisher's open access policy
ISSN 1462-2912
Publication date 2014-08-01
Year available 2015
Sub-type Article (original research)
DOI 10.1111/1462-2920.12582
Open Access Status Not yet assessed
Volume 17
Issue 5
Start page 1574
End page 1585
Total pages 12
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Subject 2404 Microbiology
1105 Ecology, Evolution, Behavior and Systematics
Abstract Enhanced biological phosphorus removal (EBPR) is an important industrial wastewater treatment process mediated by polyphosphate-accumulating organisms (PAOs). Members of the genus CandidatusAccumulibacter are one of the most extensively studied PAO as they are commonly enriched in lab-scale EBPR reactors. Members of different Accumulibacter clades are often enriched through changes in reactor process conditions; however, the two currently sequenced Accumulibacter genomes show extensive metabolic similarity. Here, we expand our understanding of Accumulibacter genomic diversity through recovery of eight population genomes using deep metagenomics, including seven from phylogenetic clades with no previously sequenced representative. Comparative genomic analysis revealed a core of shared genes involved primarily in carbon and phosphorus metabolism; however, each Accumulibacter genome also encoded a substantial number of unique genes (>700 genes). A major difference between the Accumulibacter clades was the type of nitrate reductase encoded and the capacity to perform subsequent steps in denitrification. The Accumulibacter clade IIF genomes also contained acetaldehyde dehydrogenase that may allow ethanol to be used as carbon source. These differences in metabolism between Accumulibacter genomes provide a molecular basis for niche differentiation observed in lab-scale reactors and may offer new opportunities for process optimization.
Keyword Microbiology
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID ARC-DP0773857
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Sat, 30 Aug 2014, 00:22:26 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences