Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma in vitro in response to non-typeable Haemophilus influenzae

Pizzutto, Susan J., Yerkovich, Stephanie T., Upham, John W., Hales, Belinda J., Thomas, Wayne R. and Chang, Anne B. (2014) Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma in vitro in response to non-typeable Haemophilus influenzae. PLoS One, 9 8: e104236.1-e104236.10. doi:10.1371/journal.pone.0104236


Author Pizzutto, Susan J.
Yerkovich, Stephanie T.
Upham, John W.
Hales, Belinda J.
Thomas, Wayne R.
Chang, Anne B.
Title Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma in vitro in response to non-typeable Haemophilus influenzae
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-08-11
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0104236
Open Access Status DOI
Volume 9
Issue 8
Start page e104236.1
End page e104236.10
Total pages 10
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1100 Agricultural and Biological Sciences
Abstract Chronic suppurative lung disease (CSLD) is characterized by the presence of a chronic wet or productive cough and recurrent lower respiratory infections. The aim of this study was to identify features of innate, cell-mediated and humoral immunity that may increase susceptibility to respiratory infections in children with CSLD. Because non-typeable Haemophilus influenzae (NTHi) is commonly isolated from the airways in CSLD, we examined immune responses to this organism in 80 age-stratified children with CSLD and compared their responses with 51 healthy control children. Cytokines involved in the generation and control of inflammation (IFN-γ, IL-13, IL-5, IL-10 at 72 hours and TNFα, IL-6, IL-10 at 24 hours) were measured in peripheral blood mononuclear cells challenged in vitro with live NTHi. We also measured circulating IgG subclass antibodies (IgG1 and IgG4) to two H. influenzae outer membrane proteins, P4 and P6. The most notable finding was that PBMC from children with CSLD produced significantly less IFN-c in response to NTHi than healthy control children whereas mitogen-induced IFN-γ production was similar in both groups. Overall there were minor differences in innate and humoral immune responses between CSLD and control children. This study demonstrates that children with chronic suppurative lung disease have an altered systemic cell-mediated immune response to NTHi in vitro. This deficient IFN-γ response may contribute to increased susceptibility to NTHi infections and the pathogenesis of CSLD in children.
Keyword Cystic-Fibrosis Bronchiectasis
Obstructive Pulmonary-Disease
New-Zealand
Asthma
Immunity
Sensitization
Maturation
Childhood
Cytokine
Antigen
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 1038415
13688
1040830
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Queensland Children's Medical Research Institute Publications
School of Medicine Publications
 
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